Transgene-based, female-specific lethality system for genetic sexing of the silkworm, Bombyx mori

A Tan, G Fu, L Jin, Q Guo, Z Li, B Niu… - Proceedings of the …, 2013 - National Acad Sciences
Transgene-based genetic sexing methods are being developed for insects of agricultural
and public health importance. Male-only rearing has long been sought in sericulture …

Silkworm genetic sexing through W chromosome-linked, targeted gene integration

Z Zhang, B Niu, D Ji, M Li, K Li… - Proceedings of the …, 2018 - National Acad Sciences
Sex separation methods are critical for genetic sexing systems in commercial insect
production and sterile insect techniques. Integration of selectable marker genes into a sex …

Engineered female-specific lethality for control of pest Lepidoptera

L Jin, AS Walker, G Fu, T Harvey-Samuel… - ACS synthetic …, 2013 - ACS Publications
The sterile insect technique (SIT) is a pest control strategy involving the mass release of
radiation-sterilized insects, which reduce the target population through nonviable matings. In …

Genetic sexing through the use of Y-linked transgenes

KC Condon, GC Condon, TH Dafa'alla, G Fu… - Insect biochemistry and …, 2007 - Elsevier
Sterile insect technique (SIT)-based pest control programs rely on the mass release of sterile
insects to reduce the wild target population. In many cases, it is desirable to release only …

Female-specific insect lethality engineered using alternative splicing

G Fu, KC Condon, MJ Epton, P Gong, L Jin… - Nature …, 2007 - nature.com
Abstract The Sterile Insect Technique is a species-specific and environmentally friendly
method of pest control involving mass release of sterilized insects that reduce the wild …

Transcription activator‐like effector nuclease (TALEN)‐mediated female‐specific sterility in the silkworm, Bombyx mori

J Xu, Y Wang, Z Li, L Ling, B Zeng… - Insect molecular …, 2014 - Wiley Online Library
Engineering sex‐specific sterility is critical for developing transgene‐based sterile insect
technology. Targeted genome engineering achieved by customized zinc‐finger nuclease …

Manipulating insect sex determination pathways for genetic pest management: opportunities and challenges

A Siddall, T Harvey-Samuel, T Chapman… - … in Bioengineering and …, 2022 - frontiersin.org
Sex determination pathways in insects are generally characterised by an upstream primary
signal, which is highly variable across species, and that regulates the splicing of a suite of …

Transgenic sexing system for Ceratitis capitata (Diptera: Tephritidae) based on female-specific embryonic lethality

CE Ogaugwu, MF Schetelig, EA Wimmer - Insect biochemistry and …, 2013 - Elsevier
Fruit fly pest species have been successfully controlled and managed via the Sterile Insect
Technique (SIT), a control strategy that uses infertile matings of sterile males to wild females …

Identification and characterization of a Masculinizer homologue in the diamondback moth, Plutella xylostella

T Harvey‐Samuel, VC Norman, R Carter… - Insect molecular …, 2020 - Wiley Online Library
Recently, a novel sex‐determination system was identified in the silkworm (Bombyx mori) in
which a piwi‐interacting RNA (piRNA) encoded on the female‐specific W chromosome …

Development of genetic sexing strains in Lepidoptera: from traditional to transgenic approaches

F Marec, LG Neven, AS Robinson… - Journal of Economic …, 2005 - academic.oup.com
The sterile insect technique (SIT) is currently being used for the control of many agricultural
pests, including some lepidopteran species. The SIT relies on the rearing and release of …