Genetic recombination is used as a tool for modifying the composition of poxvirus genomes
in both discovery and applied research. This review documents the history behind the …

Vaccinia virus infection results in the synthesis of a protein that promotes joint molecule
formation and strand-transfer reactionsin vitro. We show here that this activity is also …

Poxviruses encode many if not all of the proteins required for viral genome replication in the
cytoplasm of the host cell. In this context, we investigated the function of the vaccinia virus …

Poxviruses replicate in cytoplasmic structures called factories and each factory begins as a
single infecting particle. Sixty-years ago Cairns predicted that this might have effects on …

Cytoplasmic replication of poxviruses dictates the encoding of most, if not all, of the trans-
acting factors required for faithful genome duplication. Several of these proteins have been …

The duplication of the poxvirus double-stranded DNA genome occurs in cytoplasmic
membrane-delimited factories. This physical autonomy from the host nucleus suggests that …

Traditional methods for genetic manipulation of poxviruses rely on low-frequency natural
recombination in virus-infected cells. Although these powerful systems represent the …

Little is known about the mechanism of poxvirus recombination even though construction of
recombinant viruses by recombination-dependent methods is a widely adopted technique …

Poxvirus DNA polymerases play a critical role in promoting virus recombination. To test if
vaccinia polymerase (E9L) could mediate this effect by catalyzing the post-synaptic …

Poxviruses are subjected to extraordinarily high levels of genetic recombination during
infection, although the enzymes catalyzing these reactions have never been identified …