Much of our current understanding of microbiology is based on the application of genetic
engineering procedures. Since their inception (more than 30 years ago), methods based …

Repurposing the broadly distributed native CRISPR-Cas systems in prokaryotes for genome
editing is emerging as a new strategy for genetic manipulations. We recently reported the …

Despite being fundamentally important and having direct therapeutic implications, the
functional genomics of the clinical isolates of multidrug-resistant (MDR) pathogens is often …

While adoption of single‐stranded DNA recombineering techniques has greatly eased
genetic design of the platform strain Pseudomonas putida KT2440, available methods still …

The development of CRISPR-Cas-based engineering technologies has revolutionized the
microbial biotechnology field. Over the years, the Class II Type II CRISPR-Cas9 system has …

Pseudomonas aeruginosa is an antibiotic-refractory pathogen with a large genome and
extensive genotypic diversity. Historically, P. aeruginosa has been a major model system for …

Pseudomonas species have become reliable platforms for bioproduction due to their
capability to tolerate harsh conditions imposed by large‐scale bioprocesses and their …

Implementation of single‐stranded DNA (ss DNA) recombineering in Pseudomonas putida
has widened the range of genetic manipulations applicable to this biotechnologically …

CRISPR-Cas systems provide bacteria and archaea with an adaptive immune system that
targets foreign DNA. However, the xenogenic nature of immunity provided by CRISPR-Cas …

CRISPR/Cas technologies constitute a powerful tool for genome engineering, yet their use
in non-traditional bacteria depends on host factors or exogenous recombinases, which limits …