DNA repair pathway choices in CRISPR-Cas9-mediated genome editing
Many clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-
associated protein 9 (Cas9)-based genome editing technologies take advantage of Cas …
associated protein 9 (Cas9)-based genome editing technologies take advantage of Cas …
Global detection of DNA repair outcomes induced by CRISPR–Cas9
CRISPR–Cas9 generates double-stranded DNA breaks (DSBs) to activate cellular DNA
repair pathways for genome editing. The repair of DSBs leads to small insertions or …
repair pathways for genome editing. The repair of DSBs leads to small insertions or …
Precision genome editing in the CRISPR era
J Salsman, G Dellaire - Biochemistry and cell biology, 2017 - cdnsciencepub.com
With the introduction of precision genome editing using CRISPR–Cas9 technology, we have
entered a new era of genetic engineering and gene therapy. With RNA-guided …
entered a new era of genetic engineering and gene therapy. With RNA-guided …
[HTML][HTML] Understanding the diversity of genetic outcomes from CRISPR-Cas generated homology-directed repair
BM Sansbury, AM Hewes, EB Kmiec - Communications biology, 2019 - nature.com
As CRISPR-Cas systems advance toward clinical application, it is essential to identify all the
outcomes of gene-editing activity in human cells. Reports highlighting the remarkable …
outcomes of gene-editing activity in human cells. Reports highlighting the remarkable …
Covalent linkage of the DNA repair template to the CRISPR-Cas9 nuclease enhances homology-directed repair
N Savic, FCAS Ringnalda, H Lindsay, C Berk… - elife, 2018 - elifesciences.org
The CRISPR-Cas9 targeted nuclease technology allows the insertion of genetic
modifications with single base-pair precision. The preference of mammalian cells to repair …
modifications with single base-pair precision. The preference of mammalian cells to repair …
CRISPR–Cas9 genome editing in human cells occurs via the Fanconi anemia pathway
CD Richardson, KR Kazane, SJ Feng, E Zelin… - Nature …, 2018 - nature.com
CRISPR–Cas genome editing creates targeted DNA double-strand breaks (DSBs) that are
processed by cellular repair pathways, including the incorporation of exogenous DNA via …
processed by cellular repair pathways, including the incorporation of exogenous DNA via …
[HTML][HTML] Harnessing accurate non-homologous end joining for efficient precise deletion in CRISPR/Cas9-mediated genome editing
T Guo, YL Feng, JJ Xiao, Q Liu, XN Sun, JF Xiang… - Genome biology, 2018 - Springer
Background Many applications of CRISPR/Cas9-mediated genome editing require Cas9-
induced non-homologous end joining (NHEJ), which was thought to be error prone …
induced non-homologous end joining (NHEJ), which was thought to be error prone …
A glance at genome editing with CRISPR–Cas9 technology
A Barman, B Deb, S Chakraborty - Current genetics, 2020 - Springer
In recent years, CRISPR–Cas9 technology is widely acknowledged for having major
applications in the field of biotechnology for editing genome of any organism to treat a …
applications in the field of biotechnology for editing genome of any organism to treat a …
[HTML][HTML] Enhancement of precise gene editing by the association of Cas9 with homologous recombination factors
The CRISPR-Cas9 system is used for genome editing in mammalian cells by introducing
double-strand breaks (DSBs) which are predominantly repaired via non-homologous end …
double-strand breaks (DSBs) which are predominantly repaired via non-homologous end …
Pervasive head-to-tail insertions of DNA templates mask desired CRISPR-Cas9–mediated genome editing events
BV Skryabin, DM Kummerfeld, L Gubar, B Seeger… - Science …, 2020 - science.org
CRISPR-Cas9–mediated homology-directed DNA repair is the method of choice for precise
gene editing in a wide range of model organisms, including mouse and human. Broad use …
gene editing in a wide range of model organisms, including mouse and human. Broad use …