Quantitative label-free technique for morphological evaluation of human sperm—a promising tool in semen evaluation

PK Poola, V Jayaraman, K Chaithanya, D Rao… - OSA …, 2018 - opg.optica.org
We present results from non-interferometric quantitative phase imaging (QPI) for thickness
estimation and morphological analysis of human spermatozoa. QPI along with algorithms for …

Quantitative label-free sperm imaging by means of transport of intensity

PK Poola, VP Pandiyan… - … Phase Imaging II, 2016 - spiedigitallibrary.org
Most living cells are optically transparent which makes it difficult to visualize them under
bright field microscopy. Use of contrast agents or markers and staining procedures are often …

Reproductive outcomes predicted by phase imaging with computational specificity of spermatozoon ultrastructure

ME Kandel, M Rubessa, YR He… - Proceedings of the …, 2020 - National Acad Sciences
The ability to evaluate sperm at the microscopic level, at high-throughput, would be useful
for assisted reproductive technologies (ARTs), as it can allow specific selection of sperm …

Topography and refractometry of sperm cells using spatial light interference microscopy

L Liu, ME Kandel, M Rubessa… - … of biomedical optics, 2018 - spiedigitallibrary.org
Characterization of spermatozoon viability is a common test in treating infertility. Recently, it
has been shown that label-free, phase-sensitive imaging can provide a valuable alternative …

Label-free, high-throughput holographic imaging to evaluate mammalian gametes and embryos

MB Wheeler, RAC Rabel, M Rubessa… - Biology of …, 2024 - academic.oup.com
Assisted reproduction is one of the significant tools to treat human infertility. Morphological
assessment is the primary method to determine sperm and embryo viability during in vitro …

[HTML][HTML] Interferometric phase microscopy for label-free morphological evaluation of sperm cells

M Haifler, P Girshovitz, G Band, G Dardikman… - Fertility and sterility, 2015 - Elsevier
Objective To compare label-free interferometric phase microscopy (IPM) to label-free and
label-based bright-field microscopy (BFM) in evaluating sperm cell morphology. This …

Automated analysis of individual sperm cells using stain‐free interferometric phase microscopy and machine learning

SK Mirsky, I Barnea, M Levi, H Greenspan… - Cytometry Part …, 2017 - Wiley Online Library
Currently, the delicate process of selecting sperm cells to be used for in vitro fertilization
(IVF) is still based on the subjective, qualitative analysis of experienced clinicians using non …

Stain‐free interferometric phase microscopy correlation with DNA fragmentation stain in human spermatozoa

I Barnea, L Karako, SK Mirsky, M Levi… - Journal of …, 2018 - Wiley Online Library
Acridine orange (AO) staining is used to diagnose DNA fragmentation status in sperm cells.
Interferometric phase microscopy (IPM) is an optical imaging method based on digital …

Low-coherence wavelength shifting interferometry for high-speed quantitative phase imaging

S Chen, C Li, Y Zhu - Optics letters, 2016 - opg.optica.org
We propose low-coherence wavelength shifting interferometry and demonstrate its
application to quantitative phase imaging of dynamic specimens. By shifting the source …

High spatially sensitive quantitative phase imaging assisted with deep neural network for classification of human spermatozoa under stressed condition

A Butola, D Popova, DK Prasad, A Ahmad, A Habib… - Scientific reports, 2020 - nature.com
Sperm cell motility and morphology observed under the bright field microscopy are the only
criteria for selecting a particular sperm cell during Intracytoplasmic Sperm Injection (ICSI) …