Protein complexes composed of many subunits carry out most essential processes in cells
and, therefore, have become the focus of intense research. However, deciphering the …

Most eukaryotic proteins exist as large multicomponent assemblies with many subunits,
which act in concert to catalyze specific cellular activities. Many of these molecular machines …

Multiprotein complexes catalyze vital biological functions in the cell. A paramount objective
of the SPINE2 project was to address the structural molecular biology of these multiprotein …

Recombinant expression of large, multiprotein complexes is essential and often rate limiting
for determining structural, biophysical, and biochemical properties of DNA repair, replication …

Elucidation of the molecular basis of protein-interaction networks, in particular in higher
eukaryotes, is hampered by insufficient quantities of endogenous multiprotein complexes …

The capacity to reconstitute complex biological processes in vitro is a crucial step in
providing a quantitative understanding of these systems. It provides material for structural …

Multi-protein complexes are involved in essentially all cellular processes. A protein's
function is defined by a combination of its own properties, its interacting partners, and the …

We present a baculovirus-based protein engineering method that enables site-specific
introduction of unique functionalities in a eukaryotic protein complex recombinantly …

Structural and functional studies of many multiprotein complexes depend on recombinant-
protein overexpression. Rapid revision of expression experiments and diversification of the …

We present a high-throughput approach to study weak protein–protein interactions by
coupling bimolecular fluorescent complementation (BiFC) to flow cytometry (FC). In BiFC …