Although it has long been recognized that the exonucleolytic proofreading activity intrinsic to
the replicative DNA polymerases Pol δ and Pol ε is essential for faithful replication of DNA …

Efficient and accurate replication of the eukaryotic nuclear genome requires DNA
polymerases (Pols) α, δ and ɛ. In all current replication fork models, polymerase α initiates …

Measurements of nucleoside triphosphate levels in Saccharomyces cerevisiae reveal that
the four rNTPs are in 36-to 190-fold molar excess over their corresponding dNTPs. During …

Eukaryotic chromosomal DNA replication requires cyclin-dependent kinase (CDK) activity.
CDK phosphorylates two yeast replication proteins, Sld3 and Sld2, both of which bind to …

Although replication repair deficiency, either by mismatch repair deficiency (MMRD) and/or
loss of DNA polymerase proofreading, can cause hypermutation in cancer, microsatellite …

Organisms require faithful DNA replication to avoid deleterious mutations. In yeast,
replicative leading-and lagging-strand DNA polymerases (Pols ε and δ, respectively) have …

While primordial life is thought to have been RNA-based (Cech, Cold Spring Harbor
Perspect. Biol. 4 (2012) a006742), all living organisms store genetic information in DNA …

Standard of care for cancer is commonly a combination of surgery with radiotherapy or
chemoradiotherapy. However, in some advanced cancer patients this approach might still …

The mechanisms by which imbalanced dNTPs induce mutations have been well
characterized within a test tube, but not in vivo. We have examined mechanisms by which …

Translesion DNA synthesis is an important branch of the DNA damage tolerance pathway
that assures genomic integrity of living organisms. The mechanisms of DNA polymerase …