Enzymatic synthesis of fructooligosaccharides by levansucrase from Bacillus amyloliquefaciens: specificity, kinetics, and product characterization

F Tian, S Karboune - Journal of Molecular Catalysis B: Enzymatic, 2012 - Elsevier
The kinetic parameters and the product spectra of purified Bacillus amyloliquefaciens
levansucrase (LS) were investigated in the present study. LS was highly transfructosylic than …

Purification and characterization of heat-stable exo-inulinase from Streptomyces sp.

AD Sharma, PK Gill - Journal of Food Engineering, 2007 - Elsevier
The extracellular exo-inulinase from Streptomyces sp. was purified from the culture broth by
ammonium sulphate precipitation, followed by successive chromatographies on DEAE …

Application of glycosidases and transglycosidases in the synthesis of oligosaccharides

FJ Plou, AG Segura, A Ballesteros - Industrial Enzymes: Structure …, 2007 - Springer
It is generally accepted that an oligosaccharide is a carbohydrate consisting of 2–10
monosaccharide residues linked by O-glycosidic bonds (Eggleston and Cote, 2003; …

Exploring the sequence variability of polymerization-involved residues in the production of levan-and inulin-type fructooligosaccharides with a levansucrase

C Possiel, ME Ortiz-Soto, J Ertl, A Münch, A Vogel… - Scientific Reports, 2019 - nature.com
The connection between the gut microbiome composition and human health has long been
recognized, such that the host-microbiome interplay is at present the subject of the so-called …

Sugarcane (Saccharum hybrid) genetic transformation mediated by Agrobacterium tumefaciens: production of transgenic plants expressing proteins with agronomic …

GA Enriquez, LE Trujillo, C Menendez… - Developments in plant …, 2000 - Elsevier
Sugarcane is an important crop for many countries around the world. Investment in breeding
efforts remains the best approach for maximizing productivity of sugarcane. However, due to …

Impaired coordination of nucleophile and increased hydrophobicity in the+ 1 subsite shift levansucrase activity towards transfructosylation

ME Ortiz-Soto, C Possiel, J Görl, A Vogel… - …, 2017 - academic.oup.com
Abstract Bacterial levansucrases produce β (2, 6)-linked levan-type polysaccharides using
sucrose or sucrose analogs as donor/acceptor substrates. However, the dominant reaction …

High-throughput assay of levansucrase variants in search of feasible catalysts for the synthesis of fructooligosaccharides and levan

K Mardo, T Visnapuu, M Gromkova, A Aasamets… - Molecules, 2014 - mdpi.com
Bacterial levansucrases polymerize fructose residues of sucrose to β-2, 6 linked fructans—
fructooligosaccharides (FOS) and levan. While β-2, 1-linked FOS are widely recognized as …

The effect of production parameters on the synthesis of the prebiotic trisaccharide, neokestose, by Xanthophyllomyces dendrorhous (Phaffia rhodozyma)

SM Kritzinger, SG Kilian, MA Potgieter… - Enzyme and microbial …, 2003 - Elsevier
The effects of important process parameters on the production of the novel prebiotic
trisaccharide neokestose from sucrose by Xanthophyllomyces dendrorhous cells suspended …

[HTML][HTML] Catalytical properties of N-glycosylated Gluconacetobacter diazotrophicus levansucrase produced in yeast

LE Trujillo Toledo, R Gómez Riera… - Electronic Journal of …, 2004 - SciELO Chile
The influence of N-glycosylation on the kinetic and catalytical properties of a bacterial
fructosyltransferase (LsdA) produced in Pichia pastoris was studied. The glycosylated …

Fructooligosaccharides (FOS) Production by Microorganisms with Fructosyltransferase Activity

Y Belmonte-Izquierdo, LF Salomé-Abarca… - Fermentation, 2023 - mdpi.com
Fructans are fructose-based polymers, defined as fructooligosaccharides (FOS), when they
possess a short chain. These molecules are highly appreciated in the food and …