FRAP to characterize molecular diffusion and interaction in various membrane environments

F Pincet, V Adrien, R Yang, J Delacotte, JE Rothman… - PloS one, 2016 - journals.plos.org
Fluorescence recovery after photobleaching (FRAP) is a standard method used to study the
dynamics of lipids and proteins in artificial and cellular membrane systems. The advent of …

Presynaptic and postsynaptic scaffolds: dynamics fast and slow

NE Ziv, A Fisher-Lavie - The Neuroscientist, 2014 - journals.sagepub.com
The development of methods to follow the dynamics of synaptic molecules in living neurons
has radically altered our view of the synapse, from that of a generally static structure to that …

Membrane Fluidity Is Regulated Cell Nonautonomously by Caenorhabditis elegans PAQR-2 and Its Mammalian Homolog AdipoR2

R Bodhicharla, R Devkota, M Ruiz, M Pilon - Genetics, 2018 - academic.oup.com
The properties of cell membranes are determined mostly by the types of fatty acids that they
contain. Bodhicharla et al. report that a key regulator of membrane fluidity, the PAQR-2/IGLR …

Bioinformatics approaches to single-cell analysis in developmental biology

D Yalcin, ZM Hakguder, HH Otu - MHR: Basic science of …, 2016 - academic.oup.com
Individual cells within the same population show various degrees of heterogeneity, which
may be better handled with single-cell analysis to address biological and clinical questions …

PICK 1 links Argonaute 2 to endosomes in neuronal dendrites and regulates mi RNA activity

A Antoniou, M Baptista, N Carney, JG Hanley - EMBO reports, 2014 - embopress.org
MicroRNAs fine‐tune gene expression by inhibiting the translation of mRNA targets.
Argonaute (Ago) proteins are critical mediators of microRNA‐induced post‐transcriptional …

ATP binding to synaspsin IIa regulates usage and clustering of vesicles in terminals of hippocampal neurons

Y Shulman, A Stavsky, T Fedorova… - Journal of …, 2015 - Soc Neuroscience
Synaptic transmission is expensive in terms of its energy demands and was recently shown
to decrease the ATP concentration within presynaptic terminals transiently, an observation …

Novel insights in membrane biology utilizing fluorescence recovery after photobleaching

A Chattopadhyay, M Jafurulla - Biochemical Roles of Eukaryotic Cell …, 2015 - Springer
Understanding cellular signaling by membrane receptors in terms of their lateral dynamics
represents a challenging area in contemporary biology. Fluorescence recovery after …

Using FRAP or FRAPA to visualize the movement of fluorescently labeled proteins or cellular organelles in live cultured neurons transformed with adeno-associated …

Y Tevet, D Gitler - High-Resolution Imaging of Cellular Proteins: Methods …, 2016 - Springer
Fluorescence recovery after photobleaching (FRAP) and fluorescence redistribution after
photoactivation (FRAPA) are complementary methods used to gauge the movement of …

Using fluorescence recovery after photobleaching to study gap junctional communication in vitro

M Kuzma-Kuzniarska, C Yapp, PA Hulley - Gap Junction Protocols, 2016 - Springer
Fluorescence recovery after photobleaching (FRAP) is a microscopy-based technique to
study the movement of fluorescent molecules inside a cell. Although initially developed to …

[PDF][PDF] Characterisation of Virus-Induced Cytoplasmic Domains using the Herpes Simplex Virus Protein UL46

DM Perry - 2023 - openresearch.surrey.ac.uk
Cell biological studies of a range of viruses have led to the discovery of virus-induced
cellular compartments. In the case of herpes simplex virus type 1 (HSV-1), novel and …