Sample preparation: the forgotten beginning

B Brehm-Stecher, C Young, LA Jaykus… - Journal of Food …, 2009 - Elsevier
Advances in molecular technologies and automated instrumentation have provided many
opportunities for improved detection and identification of microorganisms; however, the …

Enrichment cultivation in detection of food-borne Salmonella

S Taskila, M Tuomola, H Ojamo - Food control, 2012 - Elsevier
Food-borne Salmonellosis is a significant risk to public health. Despite wide variety of rapid
molecular methods, the detection of Salmonella still requires at least one enrichment …

Human norovirus binding to select bacteria representative of the human gut microbiota

EA Almand, MD Moore, J Outlaw, LA Jaykus - PLoS One, 2017 - journals.plos.org
Recent reports describe the ability of select bacterial strains to bind human norovirus,
although the specificity of such interactions is unknown. The purpose of this work was to …

[图书][B] Handbook of Listeria monocytogenes

D Liu - 2008 - taylorfrancis.com
Once feared as a deadly intracellular bacterium with the extraordinary capacity to survive a
wide array of arduous external stressors, Listeria monocytogenes is increasingly recognized …

Detection of enterotoxigenic Staphylococcus aureus isolates in raw milk cheese

P Cremonesi, G Perez, G Pisoni… - Letters in applied …, 2007 - academic.oup.com
Aim: To develop an easy, rapid and efficient DNA extraction procedure for Staphylococcus
aureus detection with a low number of steps and removing completely the PCR inhibitors …

Direct quantitation and detection of salmonellae in biological samples without enrichment, using two-step filtration and real-time PCR

PFG Wolffs, K Glencross, R Thibaudeau… - Applied and …, 2006 - Am Soc Microbiol
ABSTRACT A new two-step filtration protocol followed by a real-time PCR assay based on
SYBR green I detection was developed to directly quantitate salmonellae in two types of …

Culture‐dependent and culture‐independent nucleic‐acid‐based methods used in the microbial safety assessment of milk and dairy products

V Fusco, GM Quero - … Reviews in Food Science and Food …, 2014 - Wiley Online Library
Despite great advances in the diagnostics and better awareness for food safety and security
worldwide, significant numbers of foodborne outbreaks have been traced back to the …

Fluorescent nanoparticles: Sensing pathogens and toxins in foods and crops

KP Burris, CN Stewart Jr - Trends in food science & technology, 2012 - Elsevier
Emerging tools in molecular nanotechnology will be useful to monitor and quantify pathogen
levels and toxin production inter-and intra-cellularly and on the surface of foods. Recent …

Detection and quantification of Campylobacter jejuni and Campylobacter coli using real‐time multiplex PCR

N Toplak, M Kovač, S Piskernik… - Journal of Applied …, 2012 - academic.oup.com
Aims: We describe a real‐time quantitative multiplex polymerase chain reaction (qmPCR)
assay to identify and discriminate between isolates of Campylobacter jejuni and …

Evaluation of paramagnetic beads coated with recombinant listeria phage endolysin–derived cell-wall-binding domain proteins for separation of Listeria …

G Walcher, B Stessl, M Wagner… - Foodborne …, 2010 - liebertpub.com
The aim of this study was to evaluate a fast and simple bead-based method using
paramagnetic beads covered with recombinant Listeria phage endolysin–derived cell-wall …