Yeast lipids have been defined in two opera-tional forms dependent upon the ease of extrac-
tion. The" readily extracted" lipids are generally best recovered (218, 232, 298) using a …

The membranes of Saccharomyces cerevisiae are composed of a typical mixture of
phospholipids which serve a general function in cellular compartmentation but may also …

Cardiolipin (CL) is a unique phospholipid which is present throughout the eukaryotic
kingdom and is localized in mitochondrial membranes. Saccharomyces cerevisiae cells …

We have identified a Saccharomyces cerevisiae gene necessary for the step in sphingolipid
synthesis in which inositol phosphate is added to ceramide to form inositol-P-ceramide, a …

Diacylglycerol esterification provides an excellent target for the pharmacological reduction of
triglyceride accumulation in several human disease states. We have usedSaccharomyces …

A purification procedure for L-myo-inositol-1-phosphate synthase (EC 5.5. 1.4) from yeast is
described. The method routinely produces enrichments of 500-fold with 20-40% yields. In …

Yeast phosphatidylinositol transfer protein (Secl4p) is required for the production of
secretory vesicles from the Golgi. This requirement can be relieved by inactivation of the …

Objectives—Atherosclerotic lesions have regions that are hypoxic. Because the lesion
contains macrophages that are loaded with lipid, we investigated whether hypoxia can …

A turnover experiment was performed with yeast cells uniformly labeled with both 32 P and
[2-3 H] inositol showing that the major inositol-containing compound phosphatidylinositol …

Using highly enriched membrane preparations from lactate-grown Saccharomyces
cerevisiae cells, the subcellular and submitochondrial location of eight enzymes involved in …