Structural and Functional Characterization of Basic PLA2 Isolated from Crotalus durissus terrificus Venom

DG Oliveira, MH Toyama, JC Novello… - Journal of protein …, 2002 - Springer
The venom of Crotalus durissus terrificus was fractionated by reverse-phase HPLC to obtain
crotapotins (F5 and F7) and PLA 2 (F15, F16, and F17) of high purity. The phospholipases A …

Isolation and Preliminary Enzymatic Characterization of a Novel PLA2 from Crotalus durissus collilineatus Venom

LA Ponce-Soto, MH Toyama, S Hyslop… - Journal of protein …, 2002 - Springer
A crotoxin homolog was purified from the Crotalus durissus collilineatus venom using
molecular exclusion and reverse-phase HPLC. This crotoxin contained one PLA 2 (Cdcolli III …

Structural, enzymatic and biological properties of new PLA2 isoform from Crotalus durissus terrificus venom

MH Toyama, DG de Oliveira, LOS Beriam, JC Novello… - Toxicon, 2003 - Elsevier
We isolated a new PLA2 from the Crotalus durissus terrificus venom that designated F15,
which showed allosteric behavior with a Vmax of 8.5 nmol/min/mg and a Km of 38.5 mM …

Enzymatic and structural characterization of new PLA2 isoform isolated from white venom of Crotalus durissus ruruima

EBS Diz Filho, S Marangoni, DO Toyama… - Toxicon, 2009 - Elsevier
This work reports the structural and enzymatic characterization of a new sPLA2 from the
white venom of Crotalus durissus ruruima, nominated PLA2A. The homogeneity of the …

[HTML][HTML] Biological and Structural Characterization of Crotoxin and New Isoform of Crotoxin B PLA2 (F6a) from Crotalus durissus collilineatus Snake Venom

LA Ponce-Soto, B Lomonte, L Rodrigues-Simioni… - The Protein …, 2007 - Springer
A new crotoxin B isoform PLA 2 (F6a), from Crotalus durissus collilineatus was purified from
by one step reverse phase HPLC chromatography using μ-Bondapack C-18 column …

Enzymatic characterization of a novel phospholipase A2 from Crotalus durissus cascavella rattlesnake (maracambóia) venom

DG Beghini, MH Toyama, S Hyslop, L Sodek… - Journal of protein …, 2000 - Springer
The PLA 2 and crotapotin subunits of crotoxin from Crotalus durissus cascavella venom
were purified by a combination of HPLC molecular exclusion (Protein Pack 300SW column) …

Biochemical, Pharmacological and Structural Characterization of a New PLA2 from Crotalus durissus terrificus (South American Rattlesnake) Venom

S Hernandez-Oliveira, MH Toyama, DO Toyama… - The Protein …, 2005 - Springer
Abstract A new PLA 2 (F16) was purified from Crotalus durissus terrificus venom by
molecular exclusion chromatography followed by analytical reverse phase HPLC. The PLA …

Isolation and enzymatic characterization of a basic phospholipase A2 from Bothrops jararacussu snake venom

VL Bonfim, MH Toyama, JC Novello, S Hyslop… - Journal of Protein …, 2001 - Springer
A novel basic phospholipase A 2 (PLA2) isoform was isolated from Bothrops jararacussu
snake venom and partially characterized. The venom was fractionated by HPLC ion …

[HTML][HTML] Biochemical, Pharmacological and Structural Characterization of Two PLA2 Isoforms Cdr-12 and Cdr-13 from Crotalus durissus ruruima Snake Venom

LA Ponce-Soto, PA Baldasso, FF Romero-Vargas… - The Protein …, 2007 - Springer
Abstract Cdr-12 and Cdr-13 isoforms of PLA 2, a D49 protein, were purified from Crotalus
durissus ruruima venom after one chromatographic step, reverse phase HPLC on μ …

Biochemical and biological characterization of a PLA2 from crotoxin complex of Crotalus durissus cumanensis

JA Pereañez, V Núñez, S Huancahuire-Vega… - Toxicon, 2009 - Elsevier
A new PLA2 (Cdcum6) from crotoxin complex of Colombian Crotalus durissus cumanensis
rattlesnake was purified using molecular exclusion chromatography and RP-HPLC. The …