Evaluation of the mecA femB Duplex Polymerase Chain Reaction for Detection of Methicillin-Resistant Staphylococcus aureus
D Jonas, H Grundmann, D Hartung… - European Journal of …, 1999 - Springer
This study systematically evaluated a recently described duplex polymerase chain reaction
test for methicillin-resistant Staphylococcus aureus with 25 different German epidemic …
test for methicillin-resistant Staphylococcus aureus with 25 different German epidemic …
Improved Methods for Detection of Methicillin-Resistant Staphylococcus aureus
S Rohrer, M Tschierske, R Zbinden… - European Journal of …, 2001 - Springer
In order to assess the performance of two detection methods, a set of 93 recent clinical
isolates of Staphylococcus aureus, including a large number of strains that demonstrated …
isolates of Staphylococcus aureus, including a large number of strains that demonstrated …
European multicentre evaluation of a commercial system for identification of methicillin-resistantStaphylococcus aureus
G Zambardi, J Fleurette, GC Schito… - European Journal of …, 1996 - Springer
A commercial system for the rapid detection of methicillin-resistant Staphylococcus aureus,
the BBL Crystal MRSA test (C-MRSA ID; Becton Dickinson, USA), was evaluated …
the BBL Crystal MRSA test (C-MRSA ID; Becton Dickinson, USA), was evaluated …
Development and evaluation of a PCR-based immunoassay for the rapid detection of methicillin-resistant Staphylococcus aureus
KJ Towner, DCS Talbot, R Curran… - Journal of medical …, 1998 - microbiologyresearch.org
A multiplex polymerase chain reaction (PCR), involving detection of the mecA and femB
genes, was combined with a novel immunoassay system capable of detecting specific PCR …
genes, was combined with a novel immunoassay system capable of detecting specific PCR …
Detection of methicillin and mupirocin resistance in staphylococcal hospital isolates with a touchdown multiplex polymerase chain reaction
B Sareyyüpoğlu, M Özyurt, T Haznedaroğlu, N Ardic - Folia microbiologica, 2008 - Springer
Abstract Staphylococcal hospital isolates (n= 166) were tested in a touchdown multiplex-
polymerase chain reaction assay for the identification of methicillin and mupirocin resistance …
polymerase chain reaction assay for the identification of methicillin and mupirocin resistance …
Rapid detection of methicillin-resistant staphylococci by multiplex PCR
AM Kearns, PR Seiders, J Wheeler, R Freeman… - Journal of Hospital …, 1999 - Elsevier
A multiplex PCR was developed to detect the coagulase gene (coa; pathognomic
ofStaphylococcus aureus) and the mecA gene (characteristically encoding for methicillin …
ofStaphylococcus aureus) and the mecA gene (characteristically encoding for methicillin …
New Quadriplex PCR Assay for Detection of Methicillin and Mupirocin Resistance and Simultaneous Discrimination of Staphylococcus aureus from Coagulase …
K Zhang, J Sparling, BL Chow, S Elsayed… - Journal of clinical …, 2004 - Am Soc Microbiol
Major challenges in diagnostic molecular microbiology are to develop a simple assay to
distinguish Staphylococcus aureus from the less virulent but clinically important coagulase …
distinguish Staphylococcus aureus from the less virulent but clinically important coagulase …
Evaluation of Molecular Assays for Rapid Detection of Methicillin-Resistant Staphylococcus aureus
S Malhotra-Kumar, L Van Heirstraeten… - Journal of clinical …, 2010 - Am Soc Microbiol
The diagnostic sensitivities of the BD GeneOhm and Cepheid Xpert assays were compared
using culture on log-serial dilutions of well-characterized methicillin-resistant …
using culture on log-serial dilutions of well-characterized methicillin-resistant …
Specific detection of methicillin-resistant Staphylococcus species by multiplex PCR
P Vannuffel, J Gigi, H Ezzedine… - Journal of clinical …, 1995 - Am Soc Microbiol
In Staphylococcus aureus, mecA and femA are the genetic determinants of methicillin
resistance. By using a multiplex PCR strategy, 310-and 686-bp regions of the mecA and …
resistance. By using a multiplex PCR strategy, 310-and 686-bp regions of the mecA and …
Evaluation of Three Molecular Assays for Rapid Identification of Methicillin-Resistant Staphylococcus aureus
P Francois, M Bento, G Renzi, S Harbarth… - Journal of clinical …, 2007 - Am Soc Microbiol
One home-developed assay and two commercial assays for the rapid identification of
methicillin-resistant Staphylococcus aureus (MRSA) were compared by use of a collection of …
methicillin-resistant Staphylococcus aureus (MRSA) were compared by use of a collection of …
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