Methicillin/oxacillin-resistant staphylococci are heterogeneous in their expression of
resistance to β-lactam agents and the test conditions have a major effect on the expression …

Four methods were compared for their abilities to detect methicillin-resistance of
Staphylococcus strains using mecA gene PCR analysis in 6 h as the gold standard. 57 …

Strains of staphylococci resistant to methicillin were identified immediately after introduction
of this drug. Methicillin-resistant strains have unusual properties, the most notable of which …

Objectives: To evaluate the performance of oxacillin, cefazolin, cefoxitin, cefotaxime and
imipenem discs; Etest for oxacillin; microdilution; agar screening plates with 2 and 6 mg/L of …

A multiplex PCR assay for detection of the staphylococcal mecA gene (the structural gene
for penicillin-binding protein 2a) was compared with agar dilution and disk diffusion …

Objective: To evaluate cefoxitin 5 and 10 μg discs for detection of methicillin resistance in
staphylococci. Methods: Six hundred and forty-one Staphylococcus aureus (261 mecA …

A DNA probe derived from the PBP 2a gene of the methicillin-resistant Staphylococcus
aureus COL was compared with phenotypic microbiologic tests for its ability to identify …

Staphylococci that acquire the mecA gene are usually resistant to β-lactam antibiotics
(methicillin or oxacillin resistance). mecA encodes a penicillin-binding protein (PBP 2a) that …

Methicillin-resistant Staphylococcus aureus strains (MRSA) are major human pathogens
responsible for nosocomial and serious community-acquired infections worldwide …

Detection of low-level oxacillin-resistant Staphylococcus aureus is a problem that needs
special attention, particularly in relation to methicillin-resistant S. aureus (MRSA) strains in …