Isothermal titration calorimetry (ITC) is now an established and invaluable method for
determining the thermodynamic constants, association constant and stoichiometry of …

Glycosyltransferases (GTases) transfer sugar moieties to proteins, lipids or existing glycan or
polysaccharide molecules. GTases form an important group of enzymes in the Golgi, where …

The β-1, 4-galactosyltransferase 7 (β4GalT7) enzyme is involved in proteoglycan synthesis.
In the presence of a manganese ion, it transfers galactose from UDP-galactose to xylose on …

Interactions between molecules on the surface of tumor cells and those on the target organ
endothelium play an important role in their arrest in an organ. Galectin-3 on the lung …

β-1, 4-Galactosyltransferase 7 (β4GalT7) is an essential enzyme in the biosynthesis of
glycosaminoglycan (GAG) chains of proteoglycans (PGs). Mammalian cells produce PGs …

Proteoglycans (PGs) are macromolecules that consist of long linear polysaccharides,
glycosaminoglycan (GAG) chains, covalently attached to a core protein by the carbohydrate …

Proteoglycans have important biological activities. To improve the overall synthetic
efficiency, a new chemoenzymatic route has been established for the proteoglycan linkage …

We have developed an operationally simple assay protocol for the identification and
evaluation of small molecular glycosyltransferase inhibitors. Wu and co-workers have …

Among glycosaminoglycan (GAG) biosynthetic enzymes, the human β1, 4-
galactosyltransferase 7 (hβ4GalT7) is characterized by its unique capacity to take over …

Derivatives of UMP (uridine monophosphate) with a fluorogenic substituent in position 5
represent a small but unique class of fluorophores, which has found important applications …