Sucrose synthase: A unique glycosyltransferase for biocatalytic glycosylation process development

K Schmölzer, A Gutmann, M Diricks, T Desmet… - Biotechnology …, 2016 - Elsevier
Abstract Sucrose synthase (SuSy, EC 2.4. 1.13) is a glycosyltransferase (GT) long known
from plants and more recently discovered in bacteria. The enzyme catalyzes the reversible …

[HTML][HTML] Design and biocatalytic applications of genetically fused multifunctional enzymes

DT Monterrey, I Ayuso-Fernández, I Oroz-Guinea… - Biotechnology …, 2022 - Elsevier
Fusion proteins, understood as those created by joining two or more genes that originally
encoded independent proteins, have numerous applications in biotechnology, from …

1, 2-cis O-Glycosylation: methods, strategies, principles

AV Demchenko - Current organic chemistry, 2003 - ingentaconnect.com
The aim of this review is to discuss the accomplishments made in the area of the
stereoselective formation of a 1, 2-cis glycosidic bond. Importance of this subject derives …

[HTML][HTML] Amplifying immunogenicity of prospective Covid-19 vaccines by glycoengineering the coronavirus glycan-shield to present α-gal epitopes

U Galili - Vaccine, 2020 - Elsevier
The many carbohydrate chains on Covid-19 coronavirus SARS-CoV-2 and its S-protein form
a glycan-shield that masks antigenic peptides and decreases uptake of inactivated virus or S …

[PDF][PDF] Self-sufficient Baeyer-Villiger monooxygenases: effective coenzyme regeneration for biooxygenation by fusion engineering

DET Pazmiño, R Snajdrova, BJ Baas… - Angewandte Chemie …, 2008 - research.rug.nl
Over the past few years, industrial interest in biocatalysts that perform selective oxidative
reactions has increased significantly.[1] Baeyer–Villiger monooxygenases (BVMOs) have …

Engineering of an artificial glycosylation pathway blocked in core oligosaccharide assembly in the yeast Pichia pastoris: production of complex humanized …

P Bobrowicz, RC Davidson, H Li, TI Potgieter… - …, 2004 - academic.oup.com
A significant percentage of eukaryotic proteins contain posttranslational modifications,
including glycosylation, which are required for biological function. However, the …

Genetic characterization of pilin glycosylation and phase variation in Neisseria meningitidis

PM Power, LF Roddam, K Rutter… - Molecular …, 2003 - Wiley Online Library
Pili of Neisseria meningitidis are a key virulence factor, being the major adhesin of this
capsulate organism and contributing to specificity for the human host. Pili are post …

Combined biosynthetic pathway for de novo production of UDP‐galactose: catalysis with multiple enzymes immobilized on agarose beads

Z Liu, J Zhang, X Chen, PG Wang - ChemBioChem, 2002 - Wiley Online Library
Regeneration of sugar nucleotides is a critical step in the biosynthetic pathway for the
formation of oligosaccharides. To alleviate the difficulties in the production of sugar …

Enzymes in the synthesis of bioactive compounds: the prodigious decades

E Garcı́a-Junceda, JF Garcı́a-Garcı́a, A Bastida… - Bioorganic & medicinal …, 2004 - Elsevier
The growing demand for enantiomerically pure pharmaceuticals has impelled research on
enzymes as catalysts for asymmetric synthetic transformations. However, the use of …

Preparation and characterization of a bifunctional aldolase/kinase enzyme: a more efficient biocatalyst for C C Bond Formation

L Iturrate, I Sánchez‐Moreno… - … A European Journal, 2010 - Wiley Online Library
A bifunctional aldolase/kinase enzyme named DLF has been constructed by gene fusion
through overlap extension. This fusion enzyme consists of monomeric fructose‐1, 6 …