Protein O-linked β-N-acetylglucosamine (O-GlcNAc) modification (O-GlcNAcylation) is a
unique monosaccharide modification discovered in the early 1980s. With the technological …

Antibody–drug conjugates (ADCs) combine the specificity of antibodies with the potency of
small molecules to create targeted drugs. Despite the simplicity of this concept, generation of …

The addition of O-linked-β-DN-acetylglucosamine (O-GlcNAc) onto serine and threonine
residues of nuclear and cytoplasmic proteins is an abundant, unique post-translational …

A new focus on glycoscience, a field that explores the structures and functions of sugars,
promises great advances in areas as diverse as medicine, energy generation, and materials …

Antibody-drug conjugates utilize the antibody as a delivery vehicle for highly potent cytotoxic
molecules with specificity for tumor-associated antigens for cancer therapy. Critical …

Antibodies are immunoglobulins that play essential roles in immune systems. All antibodies
are glycoproteins that carry at least one or more conserved N-linked oligosaccharides (N …

Monoclonal antibodies (MAbs) are the fastest growing class of human pharmaceuticals.
More than 20 MAbs have been approved and several hundreds are in clinical trials in …

Traditionally, non-specific chemical conjugations, such as acylation of amines on lysine or
alkylation of thiols on cysteines, are widely used; however, they have several shortcomings …

Sortase mediated ligation is a highly specific platform for conjugation that relies on the
specificity of the transpeptidase Sortase A (SrtA) for short peptide sequences (LPXTG and …

An enzyme-and click chemistry-mediated methodology for the site-selective radiolabeling of
antibodies on the heavy chain glycans has been developed and validated. To this end, a …