This report provides a perspective on metabolic glycoengineering methodology developed
over the past two decades that allows natural sialic acids to be replaced with chemical …

Antibodies are immunoglobulins that play essential roles in immune systems. All antibodies
are glycoproteins that carry at least one or more conserved N-linked oligosaccharides (N …

An enzyme-and click chemistry-mediated methodology for the site-selective radiolabeling of
antibodies on the heavy chain glycans has been developed and validated. To this end, a …

Protein glycosylation fundamentally impacts biological processes. Nontemplated
biosynthesis introduces unparalleled complexity into glycans that needs tools to understand …

In recent years, both site-specific bioconjugation techniques and bioorthogonal pretargeting
strategies have emerged as exciting technologies with the potential to improve the safety …

The Fc N-glycan chains of four therapeutic monoclonal antibodies (mAbs), namely, Avastin,
Rituxan, Remicade, and Herceptin, released by PNGase F, show by MALDI analysis that …

All cells are decorated with complex carbohydrate structures called glycans that serve as
ligands for glycan-binding proteins (GBPs) to mediate a wide range of biological processes …

Antibody based drugs, including IgG monoclonal antibodies, are an expanding class of
therapeutics widely employed to treat cancer, autoimmune and infectious diseases. IgG …

Glycodiversification of natural products is an effective strategy for small molecule drug
development. Recently, improved methods for chemo-enzymatic synthesis of glycosyl …

This review is the fifth update of the original review, published in 1999, on the application of
MALDI mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings …