3D map of the human corneal endothelial cell
Corneal endothelial cells (CECs) are terminally differentiated cells, specialized in regulating
corneal hydration and transparency. They are highly polarized flat cells that separate the …
corneal hydration and transparency. They are highly polarized flat cells that separate the …
Insider trading: Extracellular matrix proteins and their non‐canonical intracellular roles
AL Hellewell, JC Adams - Bioessays, 2016 - Wiley Online Library
In metazoans, the extracellular matrix (ECM) provides a dynamic, heterogeneous
microenvironment that has important supportive and instructive roles. Although the primary …
microenvironment that has important supportive and instructive roles. Although the primary …
[HTML][HTML] A ChIP on the shoulder? Chromatin immunoprecipitation and validation strategies for ChIP antibodies
FC Wardle, H Tan - F1000Research, 2015 - ncbi.nlm.nih.gov
Chromatin immunoprecipitation (ChIP) is a technique widely used in the study of epigenetics
and transcriptional regulation of gene expression. However, its antibody-centric nature …
and transcriptional regulation of gene expression. However, its antibody-centric nature …
[HTML][HTML] A novel selection strategy for antibody producing hybridoma cells based on a new transgenic fusion cell line
M Listek, A Hönow, M Gossen, K Hanack - Scientific Reports, 2020 - nature.com
The use of monoclonal antibodies is ubiquitous in science and biomedicine but the
generation and validation process of antibodies is nevertheless complicated and time …
generation and validation process of antibodies is nevertheless complicated and time …
HiBiT-qIP, HiBiT-based quantitative immunoprecipitation, facilitates the determination of antibody affinity under immunoprecipitation conditions
DC Ranawakage, T Takada, Y Kamachi - Scientific Reports, 2019 - nature.com
The affinity of an antibody for its antigen serves as a critical parameter for antibody
evaluation. The evaluation of antibody-antigen affinity is essential for a successful antibody …
evaluation. The evaluation of antibody-antigen affinity is essential for a successful antibody …
[HTML][HTML] How to avoid pitfalls in antibody use
D Pauly, K Hanack - F1000Research, 2015 - ncbi.nlm.nih.gov
Antibody use is ubiquitous in the biomedical sciences. However, determining best research
practices has not been trivial. Many commercially available antibodies and antibody …
practices has not been trivial. Many commercially available antibodies and antibody …
Affiblot: a dot blot-based screening device for selection of reliable antibodies
Z Svobodova, J Novotny, B Ospalkova… - Analytical …, 2021 - pubs.rsc.org
The key factor in the development of antibody-based assays is to find an antibody that has
an appropriate affinity, high specificity, and low cross-reactivity. However, this task is not …
an appropriate affinity, high specificity, and low cross-reactivity. However, this task is not …
Engineered ovarian cancer cell lines for validation of CAR T cell function
CE Repellin, P Ganesan, JF Alcudia… - Advanced …, 2020 - Wiley Online Library
A set of genetically engineered isogenic cell lines is developed to express either folate
receptor alpha or mesothelin, and a control cell line negative for both antigens. These cell …
receptor alpha or mesothelin, and a control cell line negative for both antigens. These cell …
Recommendations for description and validation of antibodies for research use
Recommendations for description and validation of antibodies for research use
Recommendations for description and validation of antibodies for research use J Steroid Biochem …
Recommendations for description and validation of antibodies for research use J Steroid Biochem …
Validation of the QR1 Antibody for the Evaluation of PD-L1 Expression in Non–Small Cell Lung Adenocarcinomas
N Brandone, C Mascaux, K Caselles… - Applied …, 2020 - journals.lww.com
The evaluation of Programmed cell Death Ligand 1 (PD-L1) expression in the tumor cells
with immunohistochemistry is a mandatory diagnostic step in the treatment of lung cancer. It …
with immunohistochemistry is a mandatory diagnostic step in the treatment of lung cancer. It …