Corneal endothelial cells (CECs) are terminally differentiated cells, specialized in regulating
corneal hydration and transparency. They are highly polarized flat cells that separate the …

In metazoans, the extracellular matrix (ECM) provides a dynamic, heterogeneous
microenvironment that has important supportive and instructive roles. Although the primary …

Chromatin immunoprecipitation (ChIP) is a technique widely used in the study of epigenetics
and transcriptional regulation of gene expression. However, its antibody-centric nature …

The use of monoclonal antibodies is ubiquitous in science and biomedicine but the
generation and validation process of antibodies is nevertheless complicated and time …

The affinity of an antibody for its antigen serves as a critical parameter for antibody
evaluation. The evaluation of antibody-antigen affinity is essential for a successful antibody …

The key factor in the development of antibody-based assays is to find an antibody that has
an appropriate affinity, high specificity, and low cross-reactivity. However, this task is not …

Antibody use is ubiquitous in the biomedical sciences. However, determining best research
practices has not been trivial. Many commercially available antibodies and antibody …

A set of genetically engineered isogenic cell lines is developed to express either folate
receptor alpha or mesothelin, and a control cell line negative for both antigens. These cell …

Recommendations for description and validation of antibodies for research use
Recommendations for description and validation of antibodies for research use J Steroid Biochem …

The evaluation of Programmed cell Death Ligand 1 (PD-L1) expression in the tumor cells
with immunohistochemistry is a mandatory diagnostic step in the treatment of lung cancer. It …