3D map of the human corneal endothelial cell

Z He, F Forest, P Gain, D Rageade, A Bernard… - Scientific reports, 2016 - nature.com
Corneal endothelial cells (CECs) are terminally differentiated cells, specialized in regulating
corneal hydration and transparency. They are highly polarized flat cells that separate the …

Insider trading: Extracellular matrix proteins and their non‐canonical intracellular roles

AL Hellewell, JC Adams - Bioessays, 2016 - Wiley Online Library
In metazoans, the extracellular matrix (ECM) provides a dynamic, heterogeneous
microenvironment that has important supportive and instructive roles. Although the primary …

[HTML][HTML] A ChIP on the shoulder? Chromatin immunoprecipitation and validation strategies for ChIP antibodies

FC Wardle, H Tan - F1000Research, 2015 - ncbi.nlm.nih.gov
Chromatin immunoprecipitation (ChIP) is a technique widely used in the study of epigenetics
and transcriptional regulation of gene expression. However, its antibody-centric nature …

[HTML][HTML] A novel selection strategy for antibody producing hybridoma cells based on a new transgenic fusion cell line

M Listek, A Hönow, M Gossen, K Hanack - Scientific Reports, 2020 - nature.com
The use of monoclonal antibodies is ubiquitous in science and biomedicine but the
generation and validation process of antibodies is nevertheless complicated and time …

HiBiT-qIP, HiBiT-based quantitative immunoprecipitation, facilitates the determination of antibody affinity under immunoprecipitation conditions

DC Ranawakage, T Takada, Y Kamachi - Scientific Reports, 2019 - nature.com
The affinity of an antibody for its antigen serves as a critical parameter for antibody
evaluation. The evaluation of antibody-antigen affinity is essential for a successful antibody …

[HTML][HTML] How to avoid pitfalls in antibody use

D Pauly, K Hanack - F1000Research, 2015 - ncbi.nlm.nih.gov
Antibody use is ubiquitous in the biomedical sciences. However, determining best research
practices has not been trivial. Many commercially available antibodies and antibody …

Affiblot: a dot blot-based screening device for selection of reliable antibodies

Z Svobodova, J Novotny, B Ospalkova… - Analytical …, 2021 - pubs.rsc.org
The key factor in the development of antibody-based assays is to find an antibody that has
an appropriate affinity, high specificity, and low cross-reactivity. However, this task is not …

Engineered ovarian cancer cell lines for validation of CAR T cell function

CE Repellin, P Ganesan, JF Alcudia… - Advanced …, 2020 - Wiley Online Library
A set of genetically engineered isogenic cell lines is developed to express either folate
receptor alpha or mesothelin, and a control cell line negative for both antigens. These cell …

Recommendations for description and validation of antibodies for research use

TL Rizner, H Sasano, MH Choi… - The Journal of …, 2016 - pubmed.ncbi.nlm.nih.gov
Recommendations for description and validation of antibodies for research use
Recommendations for description and validation of antibodies for research use J Steroid Biochem …

Validation of the QR1 Antibody for the Evaluation of PD-L1 Expression in Non–Small Cell Lung Adenocarcinomas

N Brandone, C Mascaux, K Caselles… - Applied …, 2020 - journals.lww.com
The evaluation of Programmed cell Death Ligand 1 (PD-L1) expression in the tumor cells
with immunohistochemistry is a mandatory diagnostic step in the treatment of lung cancer. It …