Attaining true quantitative data from WB requires that all the players involved in the
procedure are quality controlled including the user. Appropriate protein extraction method …

Tissues and organs are composed of distinct cell types that must operate in concert to
perform physiological functions. Efforts to create high-dimensional biomarker catalogs of …

Small-molecule chemical probes or tools have become progressively more important in
recent years as valuable reagents to investigate fundamental biological mechanisms and …

Multiplexed tissue imaging enables precise, spatially resolved enumeration and
characterization of cell types and states in human resection specimens. A growing number …

Western blot (WB) or immunoblot is a workhorse method. It is commonly used by biologists
for study of different aspects of protein biomolecules. In addition, it has been widely used in …

Genomics has contributed to the treatment of a fraction of cancer patients. However, there is
a need to profile the proteins that define the phenotype of cancer and its pathogenesis. The …

Western blotting (WB), also known as immunoblotting, is a well-known molecular biology
method that biologists often use to investigate many features of the protein, ranging from …

Validation of antibodies and other protein binders is a subject of pressing concern for the
research community and one which is uppermost in the minds of all who use antibodies as …

This article further discusses the reproducibility crisis in biomedical science and how poor
conduct of commercial antibodies contribute to this. In addition, the way quality data are …

A key component of efforts to address the reproducibility crisis in biomedical research is the
development of rigorously validated and renewable protein-affinity reagents. As part of the …