FISH—In Insect Chromosomes

VE Gokhman, VG Kuznetsova - Cytogenetics and Molecular …, 2022 - taylorfrancis.com
This chapter reviews the current state and future perspectives of fluorescence in situ
hybridization (FISH) in insect chromosomes. FISH in insect chromosomes is most often used …

Reprobing multicolor FISH preparations in lepidopteran chromosome

F Shibata, K Sahara, Y Naito, Y Yasukochi - Zoological science, 2009 - BioOne
Multicolor fluorescence in-situ hybridization (FISH) and subsequent reprobing of
chromosome preparations increase the number of chromosomes and/or anchor loci on the …

General protocol of FISH for Insects

AP Alves-Silva, LAC Barros, SG Pompolo - Fluorescence In Situ …, 2017 - Springer
The class Insecta comprises a widely distributed and diverse group of organisms. This
diversity also extends to cytogenetic data, such as chromosome number, sex determination …

Localization of tandemly repeated DNA sequences in beetle chromosomes by fluorescentin situ hybridization

C Juan, J Pons, E Petitpierre - Chromosome Research, 1993 - Springer
In situ hybridization to chromosomes and nuclei of Tenebrio molitor shows the massive
presence of a species-specific satellite DNA in all chromosomes and six sites of rDNA in …

[PDF][PDF] Breaking down the genome organization and karyotype differentiation through the epifluorescence microscope lens: insects and fish as models

DC Cabral-de-Mello, C Martins - … , Technology, Application and …, 2010 - researchgate.net
The use of fluorescent in situ hybridization (FISH) has allowed the generation of data
concerning the genome structure and chromosomal organization and differentiation of …

[HTML][HTML] FISHIS: Fluorescence In Situ Hybridization in Suspension and Chromosome Flow Sorting Made Easy

D Giorgi, A Farina, V Grosso, A Gennaro, C Ceoloni… - PLoS …, 2013 - journals.plos.org
The large size and complex polyploid nature of many genomes has often hampered
genomics development, as is the case for several plants of high agronomic value. Isolating …

Preparation of samples for comparative studies of arthropod chromosomes: visualization, in situ hybridization, and genome size estimation

R DeSalle, TR Gregory, JS Johnston - Methods in enzymology, 2005 - Elsevier
The ability to obtain large amounts of genomic sequence for organisms and high throughput
technology has led to a change in the thrust of research at the level of chromosomes in …

[HTML][HTML] Evolutionary cytogenetics of insects

VE Gokhman, VG Kuznetsova… - Frontiers in Ecology and …, 2022 - frontiersin.org
Modern contributions to evolutionary cytogenetics of insects are aimed at understanding the
evolutionary changes of the chromosome structure and function within different taxa and …

Laser‐assisted microdissection of polytene chromosomes from Diptera for the development of molecular markers

RJ Post, A KrÜGer, SB Somiari - Molecular ecology notes, 2006 - Wiley Online Library
The structural details visible under the light microscope have made polytene chromosomes
from Diptera much used in studies of the taxonomy, evolution and genetics of important …

[PDF][PDF] FISH as a chromosome identification strategy to delineate karyotypic evolution in vertebrates

K Srikulnath - Genomics and Genetics, 2010 - li01.tci-thaijo.org
Fluorescence in situ hybridization (FISH) is a powerful technique to directly localize cloned
DNA probes onto chromosomes. This approach is essential to delimit cytogenetic analysis …