Detection of recombinant Spike protein in the blood of individuals vaccinated against SARS‐CoV‐2: Possible molecular mechanisms

C Brogna, S Cristoni, G Marino… - PROTEOMICS …, 2023 - Wiley Online Library
Abstract Purpose The SARS‐CoV‐2 pandemic prompted the development and use of next‐
generation vaccines. Among these, mRNA‐based vaccines consist of injectable solutions of …

[PDF][PDF] Presence of viral spike protein and vaccinal spike protein in the blood serum of patients with long-COVID syndrome

K Dhuli, MC Medori, C Micheletti… - Eur Rev Med …, 2023 - europeanreview.org
OBJECTIVE: COVID-19 patients experience, in 10-20% of the cases, a prolonged long-
COVID syndrome, defined as the persistence of symptoms for at least two months after the …

[HTML][HTML] Systematic evaluation of IgG responses to SARS-CoV-2 spike protein-derived peptides for monitoring COVID-19 patients

Y Li, D Lai, Q Lei, Z Xu, F Wang, H Hou… - Cellular & Molecular …, 2021 - nature.com
Serological tests play an essential role in monitoring and combating the COVID-19
pandemic. Recombinant spike protein (S protein), especially the S1 protein, is one of the …

Flow cytometry multiplexed method for the detection of neutralizing human antibodies to the native SARS‐CoV‐2 spike protein

L Horndler, P Delgado, D Abia, I Balabanov… - EMBO molecular …, 2021 - embopress.org
A correct identification of seropositive individuals for the severe acute respiratory syndrome
coronavirus‐2 (SARS‐CoV‐2) infection is of paramount relevance to assess the degree of …

[HTML][HTML] Comparative assessment of sera from individuals after S-gene RNA-based SARS-CoV-2 vaccination with spike-protein-based and nucleocapsid-based …

A Dörschug, H Frickmann, J Schwanbeck, E Yilmaz… - Diagnostics, 2021 - mdpi.com
Due to the beginning of vaccination against COVID-19, serological discrimination between
vaccine-associated humoral response and serology-based surveillance of natural SARS …

[HTML][HTML] Clinical approach to post-acute sequelae after COVID-19 infection and vaccination

N Hulscher, BC Procter, C Wynn, PA McCullough - Cureus, 2023 - ncbi.nlm.nih.gov
The spike protein of SARS-CoV-2 has been found to exhibit pathogenic characteristics and
be a possible cause of post-acute sequelae after SARS-CoV-2 infection or COVID-19 …

Optimizing high-yield production of SARS-CoV-2 soluble spike trimers for serology assays

D Esposito, J Mehalko, M Drew, K Snead, V Wall… - Protein expression and …, 2020 - Elsevier
The SARS-CoV-2 spike trimer is the primary antigen for several serology assays critical to
determining the extent of SARS-CoV-2 exposure in the population. Until stable cell lines are …

Prospect of SARS-CoV-2 spike protein: Potential role in vaccine and therapeutic development

SK Samrat, AM Tharappel, Z Li, H Li - Virus research, 2020 - Elsevier
The recent outbreak of the betacoronavirus SARS-CoV-2 has become a significant concern
to public health care worldwide. As of August 19, 2020, more than 22,140,472 people are …

Production of trimeric SARS‐CoV‐2 spike protein by CHO cells for serological COVID‐19 testing

YB Johari, SRP Jaffé, JM Scarrott… - Biotechnology and …, 2021 - Wiley Online Library
We describe scalable and cost‐efficient production of full length, His‐tagged severe acute
respiratory syndrome coronavirus 2 (SARS‐CoV‐2) spike glycoprotein trimer by Chinese …

Development of a parallel reaction monitoring mass spectrometry assay for the detection of SARS-CoV-2 spike glycoprotein and nucleoprotein

LH Cazares, R Chaerkady, SH Samuel Weng… - Analytical …, 2020 - ACS Publications
There is an urgent need for robust and high-throughput methods for SARS-CoV-2 detection
in suspected patient samples to facilitate disease management, surveillance, and control …