A thermally reversible nanogel is used in capillary electrophoresis to create discrete regions
for a galactosyltransferase reaction and separation. The β1–4 galactosyltransferase …

A thermally responsive nanogel is used to create stationary zones of enzyme and lectin in a
separation capillary. Once patterned in the capillary, analyte is driven through the zone …

The kinetic parameters of a galactosyltransferase-catalyzed reaction were determined for
the first time using capillary zone electrophoresis (CZE) using the methylumbelliferyl (MU) …

Beckman Instruments, Inc., Fullerton, California 92634 separations were performed on a
P/ACE 5500 capillary electrophoresis system (Beckman Instruments, Inc., Received …

Glycosyltransferases are key enzymes in glycoconjugate biosynthesis, which make them
important targets for biomedical research. Among the different methodologies developed to …

Phospholipids are used as an additive in capillary electrophoresis to enhance the
separation of glycans derived from α1-acid glycoprotein, fetuin, and ribonuclease B. The …

Sialylation and sialic acid linkage in N-glycans are markers of disease but are analytically
challenging to quantify. A capillary electrophoresis method is reported that integrates a …

We have developed a nonradioactive method to assay UDP-Gal: β-d-GlcNAcβ1, 4-
galactosyltransferase (β4GalT-I) enzymatic activity. Capillary electrophoresis combined with …

A lectin-assisted enzyme-linked immunosorbent assay (ELISA)-based method using a
synthetic glycopolymer as an acceptor substrate was developed for measuring β1, 4 …

We have evaluated high-performance capillary electrophoresis (HPCE) with respect to its
suitability for use in establishing a carbohydrate-mapping database that would enable a …