Development and validation of a multiplex immunoassay for the simultaneous determination of serum antibodies to Bordetella pertussis, diphtheria and tetanus

PGM van Gageldonk, FG van Schaijk… - Journal of …, 2008 - Elsevier
To increase testing of vaccine induced humoral immunity in immune surveillance studies
and vaccine trials, a rapid and simple microsphere-based multiplex assay (pentaplex) was …

Performance of Commercial Enzyme-Linked Immunosorbent Assays for Detection of Antibodies to Bordetella pertussis

M Riffelmann, K Thiel, J Schmetz… - Journal of clinical …, 2010 - Am Soc Microbiol
Measuring antibodies to Bordetella pertussis antigens is mostly done by enzyme-linked
immunosorbent assays (ELISAs). We compared the performance of ELISA kits that were …

Collaborative study for the evaluation of enzyme-linked immunosorbent assays used to measure human antibodies to Bordetella pertussis antigens

F Lynn, GF Reed, BD Meade - Clinical Diagnostic Laboratory …, 1996 - Am Soc Microbiol
Acellular pertussis vaccines are being evaluated in multiple clinical studies, and human
immunogenicity data will likely be pivotal in the appraisal of vaccine responses between …

What to do and what not to do in serological diagnosis of pertussis: recommendations from EU reference laboratories

N Guiso, G Berbers, NK Fry, Q He, M Riffelmann… - European journal of …, 2011 - Springer
Bordetella pertussis-specific antibodies can be detected by enzyme-linked immunosorbent
assays (ELISAs) or multiplex immunoassays. Assays use purified or mixed antigens, and …

Comparison of Serological and Real-Time PCR Assays To Diagnose Bordetella pertussis Infection in 2007

P André, V Caro, E Njamkepo… - Journal of clinical …, 2008 - Am Soc Microbiol
Bacterial culture for diagnosing pertussis infection has high specificity but poor sensitivity
and is slow. Highly sensitive real-time PCR assays and single-serum pertussis serology …

International Bordetella pertussis assay standardization and harmonization meeting report. Centers for Disease Control and Prevention, Atlanta, Georgia, United …

ML Tondella, GM Carlone, N Messonnier, CP Quinn… - Vaccine, 2009 - Elsevier
An international meeting on Bordetella pertussis assay standardization and harmonization
was held at the Centers for Disease Control and Prevention (CDC), Atlanta, GA, 19–20 July …

Assessment of IgG avidity against pertussis toxin and filamentous hemagglutinin via an adapted enzyme-linked immunosorbent assay (ELISA) using ammonium …

G Almanzar, B Ottensmeier, J Liese, M Prelog - Journal of immunological …, 2013 - Elsevier
Antibody avidity, defined as the strength of binding between antibody and antigen,
represents a functional measure of affinity maturation of antibodies. Determination of the …

Clinical evaluation and validation of laboratory methods for the diagnosis of Bordetella pertussis infection: Culture, polymerase chain reaction (PCR) and anti …

AD Lee, PK Cassiday, LC Pawloski, KM Tatti… - PloS one, 2018 - journals.plos.org
Introduction The appropriate use of clinically accurate diagnostic tests is essential for the
detection of pertussis, a poorly controlled vaccine-preventable disease. The purpose of this …

Evaluation of real-time PCR for diagnosis of Bordetella pertussis infection

L Knorr, JD Fox, PAG Tilley, J Ahmed-Bentley - BMC infectious diseases, 2006 - Springer
Background Nucleic acid amplification of the IS481 region by PCR is more sensitive than
culture for detection and diagnosis of Bordetella pertussis but the assay has known cross …

Differences in avidity of IgG antibodies to pertussis toxin after acellular pertussis booster vaccination and natural infection

AM Barkoff, K Gröndahl-Yli-Hannuksela, J Vuononvirta… - Vaccine, 2012 - Elsevier
BACKGROUND: Pertussis toxin (PT) is a specific virulence factor of Bordetella pertussis and
it is included in all acellular pertussis vaccines (aP). Although immunity after infection seems …