Antibodies are indispensible research tools, yet the scientific community has not adopted
standardized procedures to validate their specificity. Here we present a strategy to …

There is a need for standardized validation methods for antibody specificity and selectivity.
Recently, five alternative validation pillars were proposed to explore the specificity of …

Antibodies are quintessential affinity reagents for the investigation and determination of a
protein's expression patterns, localization, quantitation, modifications, purification, and …

Antibodies are a primary research tool for a diverse range of experiments in biology, from
development to pathology. Their utility is derived from their ability to specifically identify …

Antibodies are critical reagents to detect and characterize proteins. It is commonly
understood that many commercial antibodies do not recognize their intended targets, but …

Immunofluorescence (IF) imaging using antibodies is widely used in biological and clinical
laboratories to map the distribution of proteins in situ. A typical IF protocol involves labeling …

Antibodies are useful tools to characterize the components of the human proteome and to
validate potential protein biomarkers discovered through various clinical proteomics efforts …

Antibodies are essential for elucidating gene function. However, affordable technology for
proteome-scale antibody generation does not exist. To address this, we developed …

Several approaches have been developed over the past decade to study the complex
interactions that occur in biological system. The ability to carry out a comprehensive genetic …

An important concern for the use of antibodies in various applications, such as western blot
(WB) or immunohistochemistry (IHC), is specificity. This calls for systematic validations using …