Fluorescence ratio imaging of cyclic AMP in single cells
SR Adams, AT Harootunian, YJ Buechler, SS Taylor… - Nature, 1991 - nature.com
FLUORESCENCEimaging is perhaps the most powerful technique currently available for
continuously observing the dynamic intracellular biochemistry of single living cells1 …
continuously observing the dynamic intracellular biochemistry of single living cells1 …
Cloning, expression, and chromosomal localization of beta-adrenergic receptor kinase 2. A new member of the receptor kinase family
JL Benovic, JJ Onorato, JL Arriza, WC Stone… - Journal of Biological …, 1991 - ASBMB
The beta-adrenergic receptor kinase (beta ARK) specifically phosphorylates the agonist-
occupied form of the beta-adrenergic and related G protein-coupled receptors. Structural …
occupied form of the beta-adrenergic and related G protein-coupled receptors. Structural …
Identifying the molecular switches that determine whether (Rp)-cAMPS functions as an antagonist or an agonist in the activation of cAMP-dependent protein kinase I
WRG Dostmann, SS Taylor - Biochemistry, 1991 - ACS Publications
Department of Chemistry, University of California, San Diego, 9500 Gilman Drive, LaJolla,
California 92093-0654 Received March 29, 1991; Revised Manuscript Received June 20 …
California 92093-0654 Received March 29, 1991; Revised Manuscript Received June 20 …
The activation of expressed cGMP‐dependent protein kinase isozymes Iα and Iβ is determined by the different amino‐termini
P Ruth, W Landgraf, A Keilbach, B May… - European journal of …, 1991 - Wiley Online Library
cDNA of bovine cGMP‐dependent protein kinase (cGMP kinase) isozymes Iα and Iβ differ
only in their amino‐terminal domains (amino acids 1–89 and 1–104, respectively). Each …
only in their amino‐terminal domains (amino acids 1–89 and 1–104, respectively). Each …
[HTML][HTML] Persistent protein kinase activation in the maintenance phase of long-term potentiation.
E Klann, SJ Chen, JD Sweatt - Journal of Biological Chemistry, 1991 - Elsevier
Long-term potentiation (LTP) of synaptic transmission in the hippocampus is a robust form of
synaptic plasticity that may contribute to mammalian memory formation. A variety of …
synaptic plasticity that may contribute to mammalian memory formation. A variety of …
[HTML][HTML] Oxidation of cysteines activates cGMP-dependent protein kinase.
W Landgraf, S Regulla, HE Meyer… - Journal of Biological …, 1991 - Elsevier
The functional significance of the oxidation/reduction state of sulfhydryl groups of cGMP-
dependent protein kinase (cGMP kinase) was studied at 30 degrees C using different metal …
dependent protein kinase (cGMP kinase) was studied at 30 degrees C using different metal …
Selective inhibition of a high affinity type IV cyclic AMP phosphodiesterase in bovine trachealis by AH 21-132: relevance to the spasmolytic and anti-spasmogenic …
MA Giembycz, PJ Barnes - Biochemical pharmacology, 1991 - Elsevier
The ability of a papaverine-derived bronchodilator, AH 21-132, to inhibit cyclic nucleotide
hydrolysis and to increase the cAMP content and the activity of cAMP-dependent protein …
hydrolysis and to increase the cAMP content and the activity of cAMP-dependent protein …
[HTML][HTML] Mutations in the autoinhibitor site of the regulatory subunit of cAMP-dependent protein kinase I. Replacement of Ala-97 and Ser-99 interferes with …
YJ Buechler, SS Taylor - Journal of Biological Chemistry, 1991 - Elsevier
Each regulatory (R) subunit of cAMP-dependent protein kinase contains an autoinhibitor site
that lies approximately 90-100 residues from the amino terminus. In order to study the …
that lies approximately 90-100 residues from the amino terminus. In order to study the …
Spectrophotometric determination of functional characteristics of protein kinases with coupled enzymatic assay
Z Technikova-Dobrova, AM Sardanelli, S Papa - FEBS letters, 1991 - Elsevier
The performance of enzyme-coupled spectrophotometric assay of protein kinases and its
merits as compared with the radioisotopic method in characterizing functional activity of …
merits as compared with the radioisotopic method in characterizing functional activity of …
A phosphatase resistant substrate for the assay of protein kinase C in crude tissue extracts
YJK Farrar, TC Vanaman, JT Slevin - Biochemical and biophysical …, 1991 - Elsevier
Protein kinase C (PKC) is routinely assayed, after it is partially purified over DEAE-cellulose
chromatography to eliminate any interfering protein kinases and phosphatases, by …
chromatography to eliminate any interfering protein kinases and phosphatases, by …