Specific amplification of 18S fungal ribosomal genes from vesicular-arbuscular endomycorrhizal fungi colonizing roots

L Simon, M Lalonde, TD Bruns - Applied and environmental …, 1992 - Am Soc Microbiol
The first DNA sequences obtained from arbuscular endomycorrhizal fungi are reported.
They were obtained by directly sequencing overlapping amplified fragments of the nuclear …

Design and testing of a plant‐specific PCR primer for ecological and evolutionary studies

KW CULLINGS - Molecular ecology, 1992 - Wiley Online Library
A polymerase chain reaction (PCR) primer, 28KJ (5‐GGCGGTAAATTCCGTCC‐3), was
developed to specifically amplify plant DNA. This primer is located approximately 250 bases …

Ribosomal DNA internal transcribed spacers are highly divergent in the phytopathogenic ascomycete Fusarium sambucinum (Gibberella pulicaris)

K O'Donnell - Current genetics, 1992 - Springer
Variation within the internal transcribed spacers (ITS1 and ITS2) and 5.8 s ribosomal DNA
gene of the heterothallic phytopathogenic filamentous fungus, Fusarium sambucinum …

Phylogeny of five fungus-like protoctistan Phytophthora species, inferred from the internal transcribed spacers of ribosomal DNA.

SB Lee, JW Taylor - Molecular Biology and evolution, 1992 - academic.oup.com
Ribosomal DNA variation was used to study evolutionary relationships among five fungal-
like protoctistan Phytophthora species. On the basis of morphological and ecological …

[图书][B] Ribosomal RNA analysis of microorganisms as they occur in nature

DM Ward, MM Bateson, R Weller, AL Ruff-Roberts - 1992 - Springer
Advances in molecular biology are now providing the means for solving long-standing
problems in microbiology. One of the best examples is the development of a rational …

Molecular Phylogeny of Northern Hemisphere Species of Armillaria

JB Anderson, E Stasovski - Mycologia, 1992 - Taylor & Francis
This study used polymerase chain reaction amplification and direct sequencing of double-
stranded DNA to compare the intergenic region between the 3′ end of the 26S gene and …

Sequence and secondary structure comparisons of ITS rDNA in mosquitoes (Diptera: Culicidae)

DM Wesson, CH Porter, FH Collins - Molecular phylogenetics and evolution, 1992 - Elsevier
Sequences of the internal transcribed spacers (ITS1 and ITS2) of the mosquito Aedes
aegypti, and the ITS2 of six related species, A. simpsoni, A. albopictus, A. vexans, A …

Rapid identification of genetic variation of ectomycorrhizal fungi by amplification of ribosomal RNA genes

B Henrion, F Tacon, F Martin - New Phytologist, 1992 - Wiley Online Library
There is a clear requirement to develop sensitive methods for detecting denned isolates of
ectomycorrhizal fungi within the complex microbial communities of natural ecosystems and …

Species-specific polymorphisms in transcribed ribosomal DNA of fivePythium species

W Chen, JW Hoy, RW Schneider - Experimental Mycology, 1992 - Elsevier
Twenty-five isolates representing fivePythium species collected from diverse hosts and
geographic origins were evaluated using polymerase chain reaction and restriction fragment …

NUCLEAR RIBOSOMAL DNA INTERNAL TRANSCRIBED SPACER REGIONS (ITS1 AND ITS2) DEFINE DISCRETE BIOGEOGRAPHIC GROUPS IN CLADOPHORA ALBIDA …

FT Bakker, JL Olsen, WT Stam… - Journal of …, 1992 - Wiley Online Library
Nucleotide sequences of the nuclear ribosomal DNA internal transcribed spacers (ITS1 and
ITS2), the 5.8 S, and short stretches of the adjacent 18S and 26S coding regions were …