Nucleotide sequence analysis of a transposon (Tn5393) carrying streptomycin resistance genes in Erwinia amylovora and other gram-negative bacteria

CS Chiou, AL Jones - Journal of bacteriology, 1993 - Am Soc Microbiol
A class II Tn3-type transposable element, designated Tn5393 and located on plasmid
pEa34 from streptomycin-resistant strain CA11 of Erwinia amylovora, was identified by its …

Tn5053, a mercury resistance transposon with integron's ends

GY Kholodii, OV Yurieva, OL Lomovskaya… - Journal of molecular …, 1993 - Elsevier
We describe a novel type of mercury resistance transposon, Tn5053, which was found in the
chromosome of a mercury-resistant Xanthomonas strain isolated from a mercury mine. An …

Secondary sites for integration mediated by the Tn21 integrase

MV Francia, F Cruz, JMG Lobo - Molecular microbiology, 1993 - Wiley Online Library
The integrase encoded by the integron of the transposon Tn21 can mediate the site‐specific
fusion of two plasmids if there is a recombination hot spot (59 bp element) in one of them …

TnMax—a versatile mini-transposon for the analysis of cloned genes and shuttle mutagenesis

R Haas, AF Kahrs, D Facius, H Allmeier, R Schmitt… - Gene, 1993 - Elsevier
A series of Tn7721-based mini-transposons (TnMax) has been developed which are
suitable for the targeting of cloned genes, shuttle mutagenesis, identification of protein …

A novel dihydrofolate reductase cassette inserted in an integron borne on a Tn21-like element

E Heikkilä, M Skurnik, L Sundström… - Antimicrobial agents …, 1993 - Am Soc Microbiol
In this study, a 498-bp dhfrXII gene coding for trimethoprim resistance was found inserted in
a cassette-like manner in the recombinationally active locus, the integron, borne on a …

Characterization of transposon Tn5086, carrying the site-specifically inserted gene dhfrVII mediating trimethoprim resistance

L Sundström, G Swedberg, O Sköld - Journal of bacteriology, 1993 - Am Soc Microbiol
Two different enteric plasmids of widely separate origins were observed to carry a new 15.3-
kb trimethoprim resistance transposon, Tn5086, also mediating resistance to mercuric ions …

Mobilization of chromosomes and nonconjugative plasmids by cointegrative mechanisms

C Reimmann, D Haas - Bacterial conjugation, 1993 - Springer
Conjugative plasmids in bacteria bring about the transfer of DNA by cell contact between a
donor and a recipient (250). Naturally occurring conjugative plasmids transfer themselves …

Specificity of DNA recognition in the nucleoprotein complex for site-specific recombination by Tn21 resolvase

SC Hall, SE Halford - Nucleic acids research, 1993 - academic.oup.com
Resolvases from Tnd-like transposons catalyse sitespecific recombination at res sites. Each
res site has 3 binding sites for resolvase, I, II, and III. The res sites in Tn3 and Tn21 have …

A mutagenesis system utilizing a Tn1722 derivative containing an Escherichia coli-specific vector plasmid: application to Pseudomonas species

T Masataka, N Teruko - Gene, 1993 - Elsevier
A novel transposon (Tn) mutagenesis system for Gram− non-enteric bacteria was developed
which allowed rapid and one-step cloning of the mutated region in Escherichia coli. The Tn …

A molecular analysis of a broad-host-range plasmid isolated from Thiobacillus ferrooxidans

DE Rawlings, RA Dorrington, J Rohrer… - FEMS microbiology …, 1993 - academic.oup.com
Abstract A 12.4-kb plasmid, pTF-FC2, that was isolated from Thiobacillus ferrooxidans and
which is capable of replication in a wide range of Gram-negative bacteria, has been …