Simultaneous identification of bacterial virulence genes by negative selection

M Hensel, JE Shea, C Gleeson, MD Jones, E Dalton… - Science, 1995 - science.org
An insertional mutagenesis system that uses transposons carrying unique DNA sequence
tags was developed for the isolation of bacterial virulence genes. The tags from a mixed …

Tracking genetically engineered microorganisms in nature

JK Jansson - Current Opinion in Biotechnology, 1995 - Elsevier
The past year has seen the continued development of methods for tracking genetically
engineered microorganisms in nature, with an emphasis on increased sensitivity, specificity …

Activation of the transcriptional regulator XylR of Pseudomonas putida by release of repression between functional domains

S Fernández, V de Lorenzo… - Molecular …, 1995 - Wiley Online Library
In the presence of toluene, xylenes and other structural analogues, the regulatory protein
XylR, of the family of transcriptional regulators which act in concert with the σ54 factor …

Site-specific deletions of chromosomally located DNA segments with the multimer resolution system of broad-host-range plasmid RP4

CS Kristensen, L Eberl, JM Sanchez-Romero… - Journal of …, 1995 - Am Soc Microbiol
The multimer resolution system (mrs) of the broad-host-range plasmid RP4 has been
exploited to develop a general method that permits the precise excision of chromosomal …

Aromatic effector activation of the NtrC-like transcriptional regulator PhhR limits the catabolic potential of the (methyl) phenol degradative pathway it controls

LC Ng, CL Poh, V Shingler - Journal of bacteriology, 1995 - Am Soc Microbiol
Pseudomonas putida P35X (NCIB 9869) metabolizes phenol and monomethylphenols via a
chromosomally encoded meta-cleavage pathway. We have recently described a 13.4-kb …