Mutagenesis of Burkholderia pseudomallei with Tn5-OT182: isolation of motility mutants and molecular characterization of the flagellin structural gene

D DeShazer, PJ Brett, R Carlyon… - Journal of …, 1997 - Am Soc Microbiol
Burkholderia pseudomallei is a human and animal pathogen in tropical regions, especially
Southeast Asia and northern Australia. Currently little is known about the genetics and …

Cyclic AMP and its receptor protein negatively regulate the coordinate expression of cholera toxin and toxin-coregulated pilus in Vibrio cholerae

K Skorupski, RK Taylor - Proceedings of the National …, 1997 - National Acad Sciences
Insertion mutations in two Vibrio cholerae genes, cya and crp, which encode adenylate
cyclase and the cyclic AMP (cAMP) receptor protein (CRP), respectively, derepressed the …

Modulation of gene expression through chromosomal positioning in Escherichia coli

C Sousa, V De Lorenzo, A Cebolla - Microbiology, 1997 - microbiologyresearch.org
Variations in expression of the nah genes of the NAH7 (naphthalene biodegradation)
plasmid of Pseudomonas putida when placed in different chromosomal locations in …

Green fluorescent protein-based reporter systems for genetic analysis of bacteria including monocopy applications

A Suarez, A Güttler, M Strätz, LH Staendner, KN Timmis… - Gene, 1997 - Elsevier
The green fluorescent protein (GFP) gene, gfp, was used to develop versatile reporter
systems for genetic analysis in, and monitoring of bacteria. This reporter system is available …

Designing recombinant Pseudomonas strains to enhance biodesulfurization

ME Gallardo, A Ferrandez, V De Lorenzo… - Journal of …, 1997 - Am Soc Microbiol
The dsz biodesulfurization cluster from Rhodococcus erythropolis IGTS8 has been
engineered under the control of heterologous broad-host-range regulatory signals to …

Effector specificity mutants of the transcriptional activator NahR of naphthalene degrading Pseudomonas define protein sites involved in binding of aromatic inducers

A Cebolla, C Sousa, V de Lorenzo - Journal of Biological Chemistry, 1997 - ASBMB
This work reports a genetic analysis of the interactions between NahR, the LysR-type
regulator of the NAH operons for biodegradation of naphthalene in Pseudomonas, and its …

Genetic characterization and expression in heterologous hosts of the 3-(3-hydroxyphenyl) propionate catabolic pathway of Escherichia coli K-12

A Ferrandez, JL Garcia, E Diaz - Journal of bacteriology, 1997 - Am Soc Microbiol
We report the complete nucleotide sequence of the gene cluster encoding the 3-(3-
hydroxyphenyl) propionate (3-HPP) catabolic pathway of Escherichia coli K-12. Sequence …

Attenuation, persistence, and vaccine potential of an Edwardsiella ictaluri purA mutant

ML Lawrence, RK Cooper, RL Thune - Infection and immunity, 1997 - Am Soc Microbiol
In this study, an adenine-auxotrophic strain of Edwardsiella ictaluri was constructed and its
virulence, tissue persistence, and vaccine efficacy were evaluated. A clone containing the …

cis-trans isomerization of unsaturated fatty acids: cloning and sequencing of the cti gene from Pseudomonas putida P8

R Holtwick, F Meinhardt, H Keweloh - Applied and environmental …, 1997 - Am Soc Microbiol
Transposon mutants of Pseudomonas putida P8 were generated by applying a mini-Tn5
mutagenesis system. The mutants obtained were checked for their ability to tolerate …

A method for construction of E. coli strains with multiple DNA insertions in the chromosome

MY Peredelchuk, GN Bennett - Gene, 1997 - Elsevier
A system for construction of E. coli strains with multiple DNA insertions in the chromosome,
based on elements of modules for site specific recombination of Tn1545 and phage λ, has …