Biomimetic dyes as affinity chromatography tools in enzyme purification
Affinity adsorbents based on immobilized triazine dyes offer important advantages
circumventing many of the problems associated with biological ligands. The main drawback …
circumventing many of the problems associated with biological ligands. The main drawback …
Monosize and non-porous p (HEMA-co-MMA) microparticles designed as dye-and metal-chelate affinity sorbents
Congo red was immobilised onto monosize and non-porous poly (2-
hydroxyethylmethacrylate-co-methylmethacrylate)[p (HEMA-co-MMA)] copolymer …
hydroxyethylmethacrylate-co-methylmethacrylate)[p (HEMA-co-MMA)] copolymer …
Dye-ligand affinity chromatography for protein separation and purification
NE Labrou - Affinity Chromatography: Methods and Protocols, 2000 - Springer
Affinity chromatography has proven to be the most effective technique for the purification and
separation of proteins from complex mixtures (1). Although affinity adsorbents based on …
separation of proteins from complex mixtures (1). Although affinity adsorbents based on …
Improved purification of Candida boidinii formate dehydrogenase
NE Labrou - Bioseparation, 2000 - Springer
Abstract Formate dehydrogenase (FDH, EC 1.2. 1.2) from Candida boidinii was purified to
homogeneity. The two step procedure comprised anion exchange chromatography (2.9-fold …
homogeneity. The two step procedure comprised anion exchange chromatography (2.9-fold …
Growth of Candida boidinii on methanol and the activity of methanol-degrading enzymes as affected from formaldehyde and methylformate
G Aggelis, N Margariti, C Kralli, F Flouri - Journal of biotechnology, 2000 - Elsevier
Formaldehyde and methylformate affect the growth of Candida boidinii on methanol and the
activity of methanol-degrading enzymes. The presence of both intermediates in the feeding …
activity of methanol-degrading enzymes. The presence of both intermediates in the feeding …
Dye affinity labelling of yeast alcohol dehydrogenase
NE Labrou - Journal of Enzyme Inhibition, 2000 - Taylor & Francis
The interaction of yeast alcohol dehydrogenase (ADH) with the reactive chlorotriazine dye
Vilmafix Blue AR (VBAR) was studied. VBAR was purified to homogeneity on lipophilic …
Vilmafix Blue AR (VBAR) was studied. VBAR was purified to homogeneity on lipophilic …