Quorum Sensing in the Plant Pathogen Erwinia carotovora subsp. carotovora: The Role of expREcc

RA Andersson, ARB Eriksson… - Molecular plant …, 2000 - Am Phytopath Society
The production of the main virulence determinants of the plant pathogen Erwinia carotovora
subsp. carotovora, the extracellular cell wall-degrading enzymes, is partly controlled by the …

Integration-proficient plasmids for Pseudomonas aeruginosa: site-specific integration and use for engineering of reporter and expression strains

TT Hoang, AJ Kutchma, A Becher, HP Schweizer - Plasmid, 2000 - Elsevier
An improved method for integration of exogenous DNA fragments at a defined site within the
genome of Pseudomonas aeruginosa was developed. The method relies on two integration …

Engineering a mouse metallothionein on the cell surface of Ralstonia eutropha CH34 for immobilization of heavy metals in soil

M Valls, S Atrian, V de Lorenzo, LA Fernández - Nature biotechnology, 2000 - nature.com
Here we describe targeting of the mouse metallothionein I (MT) protein to the cell surface of
the heavy metal-tolerant Ralstonia eutropha (formerly Alcaligenes eutrophus) CH34 strain …

Cloning and Characterization of SmeDEF, a Novel Multidrug Efflux Pump from Stenotrophomonas maltophilia

A Alonso, JL Martínez - Antimicrobial agents and chemotherapy, 2000 - Am Soc Microbiol
Stenotrophomonas maltophilia is a nosocomial bacterial pathogen intrinsically resistant to
several antibiotics. The mechanisms involved in this intrinsic multiresistance phenotype are …

DNA methylation‐dependent regulation of Pef expression in Salmonella typhimurium

B Nicholson, D Low - Molecular microbiology, 2000 - Wiley Online Library
Plasmid‐encoded fimbriae (Pef) expressed by Salmonella typhimurium mediate adhesion to
mouse intestinal epithelium. The pef operon shares features with the Escherichia coli …

Transcriptional Regulation of the Divergent paaCatabolic Operons for Phenylacetic Acid Degradation inEscherichia coli

A Ferrández, JL Garcıa, E Dıaz - Journal of Biological Chemistry, 2000 - ASBMB
The expression of the divergently transcribedpaaZ and paaABCDEFGHIJK catabolic
operons, which are responsible for phenylacetic acid (PA) degradation inEscherichia coli, is …

Enhancing desulphurization by engineering a flavin reductase‐encoding gene cassette in recombinant biocatalysts

B Galán, E Díaz, JL García - Environmental Microbiology, 2000 - Wiley Online Library
Biological desulphurization of petroleum feedstocks and products may offer an attractive
alternative to reduce sulphur oxide emissions that cause serious environmental pollution …

Proline Catabolism by Pseudomonas putida: Cloning, Characterization, and Expression of the put Genes in the Presence of Root Exudates

S Vílchez, L Molina, C Ramos, JL Ramos - Journal of Bacteriology, 2000 - Am Soc Microbiol
Pseudomonas putida KT2442 is a root-colonizing strain which can use proline, one of the
major components in root exudates, as its sole carbon and nitrogen source. A P. putida …

New virulence-activated and virulence-repressed genes identified by systematic gene inactivation and generation of transcriptional fusions in Bordetella pertussis

R Antoine, S Alonso, D Raze, L Coutte… - Journal of …, 2000 - Am Soc Microbiol
An in silico scan of the partially completed genome sequence of Bordetella pertussis and
analyses of transcriptional fusions generated with a new integrational vector were used to …

Identification and analysis of bacterial virulence genes in vivo

KE Unsworth, DW Holden - Philosophical Transactions of …, 2000 - royalsocietypublishing.org
Signature–tagged mutagenesis is a mutation–based screening method for the identification
of virulence genes of microbial pathogens. Genes isolated by this approach fall into three …