Medical molecular farming: production of antibodies, biopharmaceuticals and edible vaccines in plants

H Daniell, SJ Streatfield, K Wycoff - Trends in plant science, 2001 - cell.com
The use of plants for medicinal purposes dates back thousands of years but genetic
engineering of plants to produce desired biopharmaceuticals is much more recent. As the …

Comprehensive survey of proteins targeted by chloroplast thioredoxin

K Motohashi, A Kondoh, MT Stumpp… - Proceedings of the …, 2001 - National Acad Sciences
Possible target proteins of chloroplast thioredoxin (Trx) have been investigated in the stroma
lysate of spinach chloroplasts. For that purpose, we immobilized a mutant of m-type Trx in …

Expression of the native cholera toxin B subunit gene and assembly as functional oligomers in transgenic tobacco chloroplasts

H Daniell, SB Lee, T Panchal, PO Wiebe - Journal of molecular biology, 2001 - Elsevier
The B subunits of enterotoxigenic Escherichia coli (LTB) and cholera toxin of Vibrio cholerae
(CTB) are candidate vaccine antigens. Integration of an unmodified CTB-coding sequence …

A strategy for the identification of proteins targeted by thioredoxin

H Yano, JH Wong, YM Lee, MJ Cho… - Proceedings of the …, 2001 - National Acad Sciences
Thioredoxins are 12-kDa proteins functional in the regulation of cellular processes
throughout the animal, plant, and microbial kingdoms. Growing evidence with seeds …

Translation of chloroplast psbA mRNA is regulated by signals initiated by both photosystems II and I

T Trebitsh, A Danon - … of the National Academy of Sciences, 2001 - National Acad Sciences
Light controls the translation of several mRNAs in fully developed chloroplasts via at least
two regulatory pathways. In the first, the light signal is transduced as a thiol-mediated signal …

Heterologous complementation of yeast reveals a new putative function for chloroplast m‐type thioredoxin

E Issakidis‐Bourguet, N Mouaheb, Y Meyer… - The Plant …, 2001 - Wiley Online Library
In the chloroplast of higher plants, two types of thioredoxins (TRX), namely TRX m which
shows high similarity to prokaryotic thioredoxins and TRX f which is more closely related to …

Thioredoxin-mediated reductive activation of a protein kinase for the regulatory phosphorylation of C4-form phosphoenolpyruvate carboxylase from maize

H Saze, Y Ueno, T Hisabori, H Hayashi… - Plant and cell …, 2001 - academic.oup.com
The activity of phosphoenolpyruvate carboxylase (PEPC, EC4. 1.1. 31) for the C4
photosynthesis is known to be regulated mainly in response to light/dark transitions through …

Eukaryotic peptide deformylases. Nuclear-encoded and chloroplast-targeted enzymes in Arabidopsis

LMA Dirk, MA Williams, RL Houtz - Plant Physiology, 2001 - academic.oup.com
Abstract Arabidopsis (ecotype Columbia-0) genes, AtDEF1 and AtDEF2, represent
eukaryotic homologs of the essential prokaryotic gene encoding peptide deformylase. Both …

[HTML][HTML] Heterodimer formation between thioredoxin f and fructose 1, 6-bisphosphatase from spinach chloroplasts

Y Balmer, P Schürmann - FEBS letters, 2001 - Elsevier
Chloroplast fructose 1, 6-bisphosphatase (FBPase) is activated by reduction of a regulatory
disulfide through thioredoxin f (Trx f). In the course of this reduction a transient mixed …

The structure and function of the ferredoxin/thioredoxin system in photosynthesis

P Schürmann, BB Buchanan - Regulation of Photosynthesis, 2001 - Springer
The demonstration that thioredoxin could function in regulation grew out of CO 2
assimilation experiments initiated more than thirty years ago with isolated chloroplasts. This …