The basic features of DNA were elucidated during the half-century following the discovery of
the double helix. But it is only during the past decade that researchers have been able to …

The completion of the human genome draft has taken several years and is only the
beginning of a period in which large amounts of DNA and RNA sequence information will be …

When interacting with a single stretched DNA, many proteins modify its end-to-end distance.
This distance can be monitored in real time using various micromanipulation techniques that …

Publisher Summary This chapter describes the optical-trap force transducer that operates by
the direct measurement of light momentum. By focusing a laser beam through a microscope …

We used a multiplexed approach based on flow-stretched DNA to monitor the enzymatic
digestion of λ-phage DNA by individual bacteriophage λ exonuclease molecules. Statistical …

Abstract RNA polymerase (RNAP) transcribes DNA discontinuously, with periods of rapid
nucleotide addition punctuated by frequent pauses. We investigated the mechanism of …

Helicase-catalyzed DNA unwinding is often studied using" all or none" assays that detect
only the final product of fully unwound DNA. Even using these assays, quantitative analysis …

The ribosome is universally responsible for synthesizing proteins by translating the genetic
code transcribed in mRNA into an amino acid sequence. Ribosomes use cellular accessory …

We demonstrate the detection of nanometer-scale conformational changes of single DNA
oligomers through a micromechanical technique. The quantity monitored is the …

We have used magnetic tweezers to study in real time chaperone-mediated chromatin
assembly/disassembly at the level of single chromatin fibers. We find a strong dependence …