An efficient animal cloning technology would provide many new opportunities for livestock
agriculture, human medicine, and animal conservation. Nuclear cloning involves the …

The successful production of viable progeny following adult somatic cell nuclear transfer
(cloning) provides exciting new opportunities for basic research for investigating early …

Somatic cell nuclear transfer embryos exhibit extensive epigenetic abnormalities, including
aberrant methylation and abnormal imprinted gene expression. In this study, a thorough …

A prevailing view of cloning by somatic‐cell nuclear transfer is that reprogramming of gene
expression occurs during the first few hours after injection of the nucleus into an oocyte, that …

Cloned mammals are readily obtained by nuclear transfer using cultured somatic cells;
however, the rate of generating live offspring from the reconstructed embryos remains low. In …

Mitochondria are semiautonomous organelles that contain double-stranded (α-helix) circular
DNA molecules (mtDNAs) of about 16300 to 16500 base pairs (bp). The mtDNA encodes …

The role of mitochondria in remodeling of the donor cell nucleus in cloned animals has
gained increased attention, as mitochondria interact in direct or indirect ways with the donor …

The birth of Louise Brown, the first IVF baby, in 1978 marked a breakthrough in infertility
treatment. In recent decades, several important new techniques have been introduced. One …

The introduction of cell nuclei into enucleated recipient cells, beyond enucleation, is the
most significant stage of somatic cloning procedure. Microsurgical transfer of somatic nuclei …

In this study, we cloned mice from ES cells by a post-electrofusion MG132 treatment and
improved development of cloned embryos with a sequential cultivation protocol. When 5 μM …