Capillary-scale monolithic immunoaffinity columns for immunoextraction with in-line laser-induced fluorescence detection

RJ Hodgson, MA Brook, JD Brennan - Analytical chemistry, 2005 - ACS Publications
A bimodal meso/macroporous monolithic silica capillary column containing an entrapped
antibody was prepared by a biocompatible sol− gel process and used for nanoflow …

Selectivity features of molecularly imprinted polymers recognising the carbamate group

C Baggiani, L Anfossi, P Baravalle, C Giovannoli… - Analytica chimica …, 2005 - Elsevier
The molecular recognition properties of molecular imprinted polymers which bind the
carbamate function were studied. Functional monomers potentially able to form non …

Capillary-scale frontal affinity chromatography/MALDI tandem mass spectrometry using protein-doped monolithic silica columns

P Kovarik, RJ Hodgson, T Covey, MA Brook… - Analytical …, 2005 - ACS Publications
Frontal affinity chromatography (FAC) interfaced with electrospray mass spectrometry (ESI-
MS) has been reported as a potential method for screening of compound mixtures against …

Development of a gas chromatography silicon-based microsystem in clinical diagnostics

L Lorenzelli, A Benvenuto, A Adami, V Guarnieri… - Biosensors and …, 2005 - Elsevier
The accurate determination of biological parameters by means of rapid, on-line
measurements at low-concentrations is an important task within the fields of pharmaceutical …

Effect-directed analysis by high-performance liquid chromatography with gas-segmented enzyme inhibition

S Fabel, R Niessner, MG Weller - Journal of Chromatography A, 2005 - Elsevier
A reversed-phase high-performance liquid chromatography system with UV-detector was
equipped with an on-line acetylcholinesterase inhibition assay to achieve effect-directed …

Reduced shrinkage of sol–gel derived silicas using sugar-based silsesquioxane precursors

Y Chen, Z Zhang, X Sui, JD Brennan… - Journal of Materials …, 2005 - pubs.rsc.org
Monolithic siliceous materials were prepared, using sol–gel based methods, from mixtures
of trifunctional silanes based on sugar lactones, including silyl-modified gluconamide GLS …

Application of Immunoaffinity Column as Cleanup Tool for an Enzyme Linked Immunosorbent Assay of Phosphinothricin-N-acetyltransferase Detection in Genetically …

W Xu, K Huang, H Zhao, Y Luo - Journal of agricultural and food …, 2005 - ACS Publications
We have developed a new immunoassay method to detect genetically modified (GM) maize
and rape containing phosphinothricin-N-acetyltransferase (PAT). PAT encoded by …

Development and evaluation of C18 and immunosorbent solid-phase extraction methods prior immunochemical analysis of chlorophenols in human urine

M Nichkova, MP Marco - Analytica chimica acta, 2005 - Elsevier
Two solid-phase extraction (SPE) methods, based on hydrophobic and selective (antibody–
antigen) interactions, have been established and evaluated as clean-up methods prior the …

HPLC with laser-induced native fluorescence detection for morphine and morphine glucuronides from blood after immunoaffinity extraction

Y Hupka, J Beike, J Roegener, B Brinkmann… - International Journal of …, 2005 - Springer
A new immunoaffinity solid phase extraction of morphine and its phase II metabolites,
morphine-3-ß-D-glucuronide and morphine-6-ß-D-glucuronide is described. An …

Microplate-based screening methods for the efficient development of sandwich immunoassays

M Kiening, R Niessner, MG Weller - Analyst, 2005 - pubs.rsc.org
The selection of suitable antibodies is a critical step in immunoassay development, since the
final assay performance is predetermined by this decision to a large extent. Particularly, the …