Mitochondria are the organelles responsible for producing the majority of a cell's ATP and
also play an essential role in gamete maturation and embryo development. ATP production …

Before fertilization, chromatins of both mouse oocytes and spermatozoa contain very few
acetylated histones. Soon after fertilization, chromatins of both gametes become highly …

During differentiation, somatic cell nuclei acquire unique patterns of epigenetic
modifications, such as DNA methylation, which affect the transcriptional activity of specific …

Cloning by somatic cell nuclear transfer (SCNT) subverts sperm-mediated fertilization that
normally leads to physiological activation of the oocyte. Therefore, artificial activation is …

The objective of this work was to obtain cloned pig offspring by uterine transfer of blastocysts
produced by zona-free manipulation. We started by defining the most suitable culture media …

Mitochondrial DNA is an extranuclear genome normally maternally inherited through the
oocyte. However, the use of nuclear transfer can result in both donor cell and recipient …

Human therapeutic cloning or nuclear transfer stem cells (NTSC) to produce patient-specific
stem cells, holds considerable promise in the field of regenerative medicine. The recent …

Cloning by nuclear transfer remains inefficient but is more efficient when nuclei from
embryonic cells or embryonic stem cells (ECNT) are employed as compared with somatic …

The potential of a stem cell to differentiate into specialized cell types for restoring normal
tissue/organ function has stimulated interest in stem cell research. The methods used to …

Somatic cell nuclear transfer has successfully been used to clone several mammalian
species including the mouse, albeit with extremely low efficiency. This study investigated …