The determination of annealing temperature is a critical step in PCR design. This parameter
is typically derived from the melting temperature of the PCR primers, so for successful PCR …

Over the past two decades, significant experimental advances have increased our ability to
control and manipulate individual DNA molecules. This capability has permitted …

Hydration represents a major force governing the conformational preferences and drug
binding properties of nucleic acids. Volumetric measurements have proven useful in …

We demonstrate a method of heterogeneous vesicle binding using membrane-anchored,
single-stranded DNA that can be used over several orders of magnitude in vesicle size, as …

A recently developed tightly bound ion model can account for the correlation and fluctuation
(ie, different binding modes) of bound ions. However, the model cannot treat mixed ion …

Using optical tweezers, we have measured the effect of monovalent cation concentration
and species on the folding free energy of five large (49− 124 nt) RNA hairpins, including HIV …

Amplicon melting is a closed-tube method for genotyping that does not require probes, real-
time analysis, or allele-specific polymerase chain reaction. However, correct differentiation …

Methylation-specific polymerase chain reaction (PCR)(MSP) is frequently used to study
gene silencing by promoter hypermethylation. However, non-specific primer design can lead …

Piezoelectric-excited, millimeter-sized cantilever (PEMC) sensors having high-mode
resonance near 1 MHz are shown to exhibit mass change sensitivity of 1− 300 ag/Hz. Gold …

The association of dicationic polycyclic ligands, namely, four diazoniapentaphene
derivatives, three diazoniaanthra [1, 2-a] anthracenes, diazoniahexaphene, and a partly …