Recombineering: in vivo genetic engineering in E. coli, S. enterica, and beyond

JA Sawitzke, LC Thomason, N Costantino… - Methods in …, 2007 - Elsevier
Abstract “Recombineering,” in vivo genetic engineering with short DNA homologies, is
changing how constructs are made. The methods are simple, precise, efficient, rapid, and …

Gene targeting in filamentous fungi: the benefits of impaired repair

S Krappmann - Fungal biology reviews, 2007 - Elsevier
The extent of published fungal genome sequences call for sophisticated genetic tools to
address the functions and features of annotated gene products. Gene targeting is the …

[HTML][HTML] CEACAM6 acts as a receptor for adherent-invasive E. coli, supporting ileal mucosa colonization in Crohn disease

N Barnich, FA Carvalho, AL Glasser… - The Journal of …, 2007 - Am Soc Clin Investig
The ileal mucosa of Crohn disease (CD) patients is abnormally colonized by adherent-
invasive E. coli (AIEC) that are able to adhere to and invade intestinal epithelial cells. Here …

Reducing mitochondrial fission results in increased life span and fitness of two fungal ageing models

CQ Scheckhuber, N Erjavec, A Tinazli, A Hamann… - Nature cell …, 2007 - nature.com
Ageing of biological systems is accompanied by alterations in mitochondrial morphology,
including a transformation from networks and filaments to punctuate units. The significance …

Construction of a Vibrio splendidus Mutant Lacking the Metalloprotease Gene vsm by Use of a Novel Counterselectable Suicide Vector

F Le Roux, J Binesse, D Saulnier… - Applied and …, 2007 - Am Soc Microbiol
Vibrio splendidus is a dominant culturable Vibrio in seawater, and strains related to this
species are also associated with mortality in a variety of marine animals. The determinants …

Characterization of lptA and lptB, Two Essential Genes Implicated in Lipopolysaccharide Transport to the Outer Membrane of Escherichia coli

P Sperandeo, R Cescutti, R Villa… - Journal of …, 2007 - Am Soc Microbiol
The outer membrane (OM) of gram-negative bacteria is an asymmetric lipid bilayer that
protects the cell from toxic molecules. Lipopolysaccharide (LPS) is an essential component …

Functional Analysis of Antigen 43 in Uropathogenic Escherichia coli Reveals a Role in Long-Term Persistence in the Urinary Tract

GC Ulett, J Valle, C Beloin, O Sherlock… - Infection and …, 2007 - Am Soc Microbiol
Escherichia coli is the primary cause of urinary tract infection (UTI) in the developed world.
The major factors associated with the virulence of uropathogenic E. coli (UPEC) are fimbrial …

Chromosomal toxin–antitoxin loci can diminish large‐scale genome reductions in the absence of selection

S Szekeres, M Dauti, C Wilde, D Mazel… - Molecular …, 2007 - Wiley Online Library
Superintegrons (SIs) are chromosomal genetic elements containing assemblies of genes,
each flanked by a recombination sequence (attC site) targeted by the integron integrase. SIs …

OmpC and the σE regulatory pathway are involved in adhesion and invasion of the Crohn's disease‐associated Escherichia coli strain LF82

N Rolhion, FA Carvalho… - Molecular …, 2007 - Wiley Online Library
Summary Ileal lesions of 36.4% of patients with Crohn's disease (CD), an inflammatory
bowel disease in humans, are colonized by pathogenic adherent‐invasive Escherichia coli …

Functional characterization of the Aspergillus fumigatus calcineurin

ME da Silva Ferreira, T Heinekamp, A Härtl… - Fungal Genetics and …, 2007 - Elsevier
Aspergillus fumigatus is an aggressive opportunistic pathogen of humans as well as a major
allergen. Environmental sensing and retrieving essential nutrients from the environment are …