Transgenic animals have become a key tool in functional genomics to generate models for
human diseases and validate new drugs. Transgenesis includes the addition of foreign …

To study the biodistribution of MSCs, we labeled adult murine C57BL/6 MSCs with firefly
luciferase and DsRed2 fluorescent protein using nonviral Sleeping Beauty transposons and …

From among a plethora of various gene delivery methods, the researcher must choose the
right one according to availability for a given species and the precise application the …

The transposon piggyBac from the genome of the cabbage looper moth Trichoplusia ni has
been observed in the laboratory to jump into the genomes of key model and pathogenic …

The Sleeping Beauty (SB) transposon system mediates chromosomal integration and stable
gene expression when an engineered SB transposon is delivered along with transposase …

A significant barrier to exploiting the full potential of the rat as a biomedical model is the lack
of tools to easily modify its germline. Here we show that a tyrosinase-tagged Sleeping …

To meet the increasing demand of linking sequence information to gene function in
vertebrate models, genetic modifications must be introduced and their effects analyzed in an …

Background Swine is an important agricultural commodity and biomedical model.
Manipulation of the pig genome provides opportunity to improve production efficiency …

Swine production has been an important part of our lives since the late Mesolithic or early
Neolithic periods, and ranks number one in world meat production. Pig production also …

Abstract The Sleeping Beauty (SB) transposon is an integrative nonviral plasmid system.
Here, we describe a protocol for SB-mediated transgene delivery using DNA …