If the atmosphere of the early Earth was hydrogen-rich, 1 it is reasonable to think that
hydrogenases, the enzymes enabling cells to use molecular hydrogen, were probably …

Hydrogenases catalyze the reversible oxidation of dihydrogen. Catalysis occurs at bimetallic
active sites that contain either nickel and iron or only iron and the nature of these active sites …

To utilize fermentative bacteria for producing the alternative fuel hydrogen, we performed
successive rounds of P1 transduction from the Keio Escherichia coli K-12 library to introduce …

Hydrogenases are metalloenzymes subdivided into two classes that contain iron-sulfur
clusters and catalyze the reversible oxidation of hydrogen gas (H 2⇆ 2H++ 2e−). Two metal …

A soluble hydrogenase from Allochromatium vinosum was purified. It consisted of a large (M
r= 52 kDa) and a small (M r= 23 kDa) subunit. The genes encoding for both subunits were …

Escherichia coli is able to grow under anaerobic fermentation conditions upon a decrease in
redox potential (Eh). Indeed, upon a transition of E. coli MC4100 wild-type culture to …

Monovalent cation/proton antiporters that catalyse electrogenic uptake of H+ in exchange for
cytoplasmic K+ and/or Na+ are centrally involved in bacterial pH homeostasis under alkaline …

Nickel and cobalt are essential micronutrients for many microorganisms and serve as
enzyme cofactors that catalyze a diverse array of reactions. One complication is that high …

[NiFe] hydrogenases are metalloenzymes involved in many biological processes concerning
the metabolism of hydrogen. The maturation of the large subunit of these hydrogenases …

It is found that the probiotic strains of Escherichia coli, G35 N 59 isolated from “Okarin” and
from “ASAP” drug formulas, and the commensal strain 5–1 isolated from healthy human …