An improved, versatile shuttle vector has been created for the metabolic engineering of
Geobacillus spp. As kanamycin is the most thermo-tolerant of commonly used antibiotics, the …

Superfolder GFP (sGFP) is a variant of the Green Fluorescent Protein that folds efficiently
when fused to poorly folded proteins. In this study, we show that sGFP, but not enhanced …

The bc1 respiratory complex III constitutes a key energy‐conserving respiratory electron
transporter between complex I (type I NADH dehydrogenase) and II (succinate …

Proteins belonging to the Omp85 family are involved in the assembly of β-barrel outer
membrane proteins or in the translocation of proteins across the outer membrane in …

Biotechnology needs to explore the capacity of different organisms to overproduce proteins
of interest at low cost. In this paper, we show that Streptomyces lividans is a suitable host for …

Genetic engineering has recently emerged as a popular tool for tailoring biological
macromolecules to function in nonnative environments. Most protein optimization efforts …

4.2. 1 Búsqueda de nuevos marcadores de selección 53 4.2. 2 Aislamiento de mutantes
resistentes a estreptomicina 53 4.2. 3 Aislamiento de las mutaciones simples del alelo rpsL1 …