Lipase nanogel catalyzed transesterification in anhydrous dimethyl sulfoxide

J Ge, D Lu, J Wang, Z Liu - Biomacromolecules, 2009 - ACS Publications
The present work showed that Candida rugosa lipase, which is inactive in anhydrous
dimethyl sulfoxide (DMSO), has been granted its original catalytic activity and greatly …

Recombinant Candida rugosa LIP2 expression in Pichia pastoris under the control of the AOX1 promoter

P Ferrer, M Alarcón, R Ramón, MD Benaiges… - Biochemical …, 2009 - Elsevier
The LIP2 isoenzyme gene from Candida rugosa has been completely synthesised and
functionally expressed under the AOX1 promoter control in Pichia pastoris. The on-line …

Study of a sterol esterase secreted by Ophiostoma piceae: sequence, model and biochemical properties

O Calero-Rueda, V Barba, E Rodríguez, F Plou… - … et Biophysica Acta (BBA …, 2009 - Elsevier
An extracellular sterol esterase from Ophiostoma piceae efficiently hydrolyzes sterol esters,
triglycerides and p-nitrophenol esters. cDNA was screened with a probe obtained by PCR …

Effects of Alcohol and Buffer Treatments on the Activity and Enantioselectivity of Candida rugosa Lipase

S Takaç, A Ezgi Ünlü - Preparative Biochemistry & Biotechnology, 2009 - Taylor & Francis
Several treatments were employed on Candida rugosa lipase (CRL) to improve its
biocatalytic performance. Besides conventional alcohol treatment conditions, the effects of …

Media Formulation using Complex Organic Nutrients for Improved Activity, Productivity, and Yield of Candida rugosa Lipase and Esterase Enzymes

S Takac, B Erdem - Preparative Biochemistry & Biotechnology, 2009 - Taylor & Francis
Candida rugosa is an excellent source of multiple lipase and esterase enzymes; therefore, it
is of technological importance to formulate the medium that provides high activity for each …

Impact of Inoculation Strategy on the Progress of Candida rugosa Cultivation

S Takaç, B Erdem, AE Ünlü - Artificial cells, blood substitutes, and …, 2009 - Taylor & Francis
Batch cultivation of Candida rugosa for the production of lipase and esterase enzymes was
carried out by employing two different inoculation strategies. The effects of triolein (2, 3 and …