Mass spectrometry (MS) is the most comprehensive and versatile tool in large-scale
proteomics. In this review, we dissect the overall framework of the MS experiment into its key …

Fast-transmitting vertebrate axons are electrically insulated with multiple layers of
nonconductive plasma membrane of glial cell origin, termed myelin. The myelin membrane …

This review is the continuation of three previously published articles [Jorrin JV, Maldonado
AM, Castillejo MA. Plant proteome analysis: a 2006 update. Proteomics 2007; 7: 2947 …

Proteomics can be defined as the large‐scale analysis of proteins. Due to the complexity of
biological systems, it is required to concatenate various separation techniques prior to mass …

The proteome project, initiated in 1995, was made possible by 2‐DE combined with MS. The
project main objective was and remains the identification of all proteins expressed by a cell …

Root hairs are single tubular cells formed from the differentiation of epidermal cells on roots.
They are involved in water and nutrient uptake and represent the infection site on …

Zymography, the detection of enzymatic activity on gel electrophoresis, has been a
technique described in the literature for at least in the past 50 years. Although a diverse …

The analysis of highly hydrophobic proteins is still an analytical challenge. Using a
recombinant gamma-aminobutyric acid A (GABAA)-receptor subunit as a model protein, we …

At first, a short history of the beginning of 2‐DE is provided. Based on the present state of the
art at the time I developed a 2‐DE technique in 1975 that was able to resolve complex …

A combination of techniques to separate and quantify the native proteins associated with a
particular transition metal ion from a cellular system has been developed. The procedure …