The old yellow enzyme (OYE) family is a large group of flavin‐dependent redox biocatalysts
with major applications in the industrial reduction of activated alkenes. These enzymes have …

Cell-free synthetic pathway biotransformation (SyPaB) is the implementation of complicated
biochemical reactions by in vitro assembling a number of enzymes or their complexes and …

Biocatalysis (based on either enzymes or whole microorganisms) has matured to a standard
technology in the finechemicals industry, as reflected in the number of biotransformation …

We report the crystal structure of a thermophilic “ene” reductase (TOYE) isolated from
Thermoanaerobacter pseudethanolicus E39. The crystal structure reveals a tetrameric …

This work describes the development of an automated robotic platform for the rapid
screening of enzyme variants generated from directed evolution studies of pentraerythritol …

Nitrile and amide bioconversions have received attention through their ability to provide a
range of commercially important chemicals. These bioconversions are mediated by distinct …

2-Keto-3-deoxygluconate aldolase from the hyperthermophile Sulfolobus solfataricus is a
highly thermostable type I aldolase that can catalyze carbon− carbon bond formation using …

The use of reagents supported on inorganic carriers is becoming increasingly more common
due to their high efficiency, which is mainly caused by a combination of several factors: a) an …

Galactitol 2-dehydrogenase (GatDH) belongs to the protein superfamily of short-chain
dehydrogenases. As an enzyme capable of the stereo-and regioselective modification of …

Protein expression level control is always a great challenge in the field of enzyme evolution.
The performance of a dedicated strain of the yeast Yarrowia lipolytica was evaluated. This …