Pharmacoproteomics: a chess game on a protein field

A D'Alessandro, L Zolla - Drug discovery today, 2010 - Elsevier
The application of proteomics in the field of drug discovery development and the
assessment of drug administration is known as pharmacoproteomics. As a branch of …

[HTML][HTML] Ocular proteomics with emphasis on two-dimensional gel electrophoresis and mass spectrometry

N Mandal, S Heegaard, JU Prause, B Honoré… - Biological procedures …, 2010 - Springer
The intention of this review is to provide an overview of current methodologies employed in
the rapidly developing field of ocular proteomics with emphasis on sample preparation, two …

[HTML][HTML] Evaluation of chloroform/methanol extraction to facilitate the study of membrane proteins of non-model plants

A Vertommen, B Panis, R Swennen, SC Carpentier - Planta, 2010 - Springer
Membrane proteins are of great interest to plant physiologists because of their important
function in many physiological processes. However, their study is hampered by their low …

2D electrophoresis-based expression proteomics: a microbiologist's perspective

I Sá-Correia, MC Teixeira - Expert Review of Proteomics, 2010 - Taylor & Francis
Quantitative proteomics based on 2D electrophoresis (2-DE) coupled with peptide mass
fingerprinting is still one of the most widely used quantitative proteomics approaches in …

Variations on a theme: changes to electrophoretic separations that can make a difference

T Rabilloud - Journal of proteomics, 2010 - Elsevier
Electrophoretic separations of proteins are widely used in proteomic analyses, and rely
heavily on SDS electrophoresis. This mode of separation is almost exclusively used when a …

Proteome and metabolome alterations in heart and liver indicate compromised energy production during sepsis

J Hinkelbein, A Kalenka, C Schubert… - Protein and Peptide …, 2010 - ingentaconnect.com
During the course of sepsis, heart and liver dysfunction occurs in 20-30% of patients. Both
septic cardiomyopathy and septic liver dysfunction have a high mortality and the underlying …

Other than IPG‐DALT: 2‐DE variants

I Miller, I Eberini, E Gianazza - Proteomics, 2010 - Wiley Online Library
Special problems require special solutions. That is why, despite the trend towards
standardization of the procedures in proteomic investigations, a number of alternative …

Quantitative Analysis of Cell Surface Membrane Proteins Using Membrane-Impermeable Chemical Probe Coupled with 18O Labeling

H Zhang, RN Brown, WJ Qian, ME Monroe… - Journal of proteome …, 2010 - ACS Publications
We report a mass spectrometry-based strategy for quantitative analysis of cell surface
membrane proteome changes. The strategy includes enrichment of surface membrane …

Quantitative proteomic approaches and their application in the study of yeast stress responses

A Szopinska, P Morsomme - Omics: a journal of integrative biology, 2010 - liebertpub.com
The increasing popularity of proteomics has created a need for quantitative analysis
methods. As a result, many different techniques are now available for performing gel-based …

Proteomics analysis of plasma membrane from liver sinusoidal endothelial cells after partial hepatectomy by an improved two-dimensional electrophoresis

X Li, L Xiong, C Xie, J Cao, H Deng, Y Lin… - Molecular and cellular …, 2010 - Springer
Liver regeneration is an angiogenesis-associated phenomenon. To identify key plasma
membrane (PM) proteins of endothelial cells involved in the initiation of angiogenesis during …