Despite their name, synonymous mutations have significant consequences for cellular
processes in all taxa. As a result, an understanding of codon bias is central to fields as …

Fluorescence has long been recognized as a powerful tool for probing biological structure
and function. Nearly sixty-five years ago, Coons and colleagues introduced the first …

Autologous expression of recombinant human proteins in human cells for biomedical
research and product development is often hampered by low expression yields limiting …

Since the discovery of the first red fluorescent protein (RFP), named DsRed, 12 years ago, a
wide pallet of red-shifted fluorescent proteins has been cloned and biotechnologically …

Purpose We describe a novel strategy for expression of GFP in mammalian mitochondria.
Methods The key components of the strategy were an artificially created mitochondrial …

Single-molecule (SM) microscopy allows outstanding insight into biomolecular mechanisms
in cells. However, selective detection of single biomolecules in their native environment …

We use a novel technique that allows for closed recirculation of vector genomes in the
cardiac circulation using cardiopulmonary bypass, referred to here as molecular cardiac …

Imaging with fluorescent proteins has been revolutionary and has led to the new field of in
vivo cell biology. Many new applications of this technology have been developed. Green …

Human parvovirus B19 (B19V) DNA is highly prevalent in endothelial cells lining up
intramyocardial arterioles and postcapillary venules of patients with chronic myocarditis and …

To investigate the codon usage pattern of the contexts flanking 11 cleavage sites of foot-and-
mouth disease virus (FMDV) polyprotein, the codon usage model of the corresponding …