Hydrogenases are metalloenzymes that are key to energy metabolism in a variety of
microbial communities. Divided into three classes based on their metal content, the [Fe] …

Cyanobacteria have tremendous potential to produce clean, renewable fuel in the form of
hydrogen gas derived from solar energy and water. Of the two cyanobacterial enzymes …

Hydrogenases are abundant enzymes that catalyse the reversible interconversion of H2 into
protons and electrons at high rates. Those hydrogenases maintaining their activity in the …

Abstract Membrane-bound respiratory [NiFe]-hydrogenase (MBH), a H2-uptake enzyme
found in the periplasmic space of bacteria, catalyses the oxidation of dihydrogen: H2→ …

An important clue to the mechanism for O2 tolerance of certain [NiFe]-hydrogenases is the
conserved presence of a modified environment around the iron–sulfur cluster that is …

Irreversible inhibition by molecular oxygen (O 2) complicates the use of [FeFe]-
hydrogenases (HydA) for biotechnological hydrogen (H 2) production. Modification by O 2 of …

Protein film electrochemistry (PFE) was utilized to characterize the catalytic activity and
oxidative inactivation of a bidirectional [NiFe]-hydrogenase (HoxEFUYH) from the …

The H2-evolving potential of [FeFe] hydrogenases is severely limited by the oxygen
sensitivity of this class of enzymes. Recent experimental studies on hydrogenase from C …

The oxygen-tolerant membrane-bound [NiFe]-hydrogenase (MBH) from Ralstonia eutropha
H16 consists of three subunits. The large subunit HoxG carries the [NiFe] active site, and the …

Two complementary experimental approaches were utilized to examine the extent to which
free soil hydrogenases and H2-oxidizing bacteria contribute to the soil uptake of …