PFunkel: efficient, expansive, user-defined mutagenesis
E Firnberg, M Ostermeier - PloS one, 2012 - journals.plos.org
We introduce PFunkel, a versatile method for extensive, researcher-defined DNA
mutagenesis using a ssDNA or dsDNA template. Once the template DNA is prepared, the …
mutagenesis using a ssDNA or dsDNA template. Once the template DNA is prepared, the …
Structural basis for efficient chromophore communication and energy transfer in a constructed didomain protein scaffold
JAJ Arpino, H Czapinska, A Piasecka… - Journal of the …, 2012 - ACS Publications
The construction of useful functional biomolecular components not currently part of the
natural repertoire is central to synthetic biology. A new light-capturing ultra-high-efficiency …
natural repertoire is central to synthetic biology. A new light-capturing ultra-high-efficiency …
A transposase strategy for creating libraries of circularly permuted proteins
MM Mehta, S Liu, JJ Silberg - Nucleic acids research, 2012 - academic.oup.com
A simple approach for creating libraries of circularly permuted proteins is described that is
called PERMutation Using Transposase Engineering (PERMUTE). In PERMUTE, the …
called PERMutation Using Transposase Engineering (PERMUTE). In PERMUTE, the …
A method for multi-codon scanning mutagenesis of proteins based on asymmetric transposons
J Liu, TA Cropp - Protein Engineering, Design & Selection, 2012 - academic.oup.com
Random mutagenesis followed by selection or screening is a commonly used strategy to
improve protein function. Despite many available methods for random mutagenesis, nearly …
improve protein function. Despite many available methods for random mutagenesis, nearly …
Experimental methods for scanning unnatural amino acid mutagenesis
J Liu, TA Cropp - Unnatural Amino Acids: Methods and Protocols, 2012 - Springer
Site-specific incorporation of unnatural amino acids into proteins in vivo relies on the genetic
reassignment of nonsense or quadruplet codons. Here, we describe a general procedure for …
reassignment of nonsense or quadruplet codons. Here, we describe a general procedure for …
[图书][B] The development and application of transposon mutagenesis and unnatural amino acid mutagenesis technologies
J Liu - 2012 - search.proquest.com
Recent three decades have seen a significant progress in the protein engineering field.
Protein engineering has not only facilitated our understanding of the sequence-structure …
Protein engineering has not only facilitated our understanding of the sequence-structure …