In just seven years, next-generation technologies have reduced the cost and increased the
speed of DNA sequencing by four orders of magnitude, and experiments requiring many …

Studies using high-resolution genome-wide approaches have recently reported that
genomic and epigenomic alterations frequently accumulate in human pluripotent cells …

Exome sequencing studies of autism spectrum disorders (ASDs) have identified many de
novo mutations but few recurrently disrupted genes. We therefore developed a modified …

The development of iPSCs reflected the merging of three major scientific streams and has in
turn led to additional new branches of investigation. However, there is still debate about …

Genome-wide dynamic changes in DNA methylation are indispensable for germline
development and genomic imprinting in mammals. Here, we report single-base resolution …

Metabolism is vital to every aspect of cell function, yet the metabolome of induced pluripotent
stem cells (iPSCs) remains largely unexplored. Here we report, using an untargeted …

The state of a cell is defined by the genes it transcribes and the epigenetic landscape that
regulates their expression. Pluripotent cells have markedly different epigenetic signatures …

Generation of human induced pluripotent stem cells (hiPSCs) by the expression of specific
transcription factors depends on successful epigenetic reprogramming to a pluripotent state …

We have adapted transposase-based in vitro shotgun library construction (“tagmentation”)
for whole-genome bisulfite sequencing. This method, Tn5mC-seq, enables a> 100-fold …

DNA methylation, consisting of the addition of a methyl group at the fifth‐position of cytosine
in a CpG dinucleotide, is one of the most well‐studied epigenetic mechanisms in mammals …